Asian-Australasian Journal of Animal Sciences

  • 제17권3호
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  • Pages.423-427
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  • 2004
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Molecular Detection of Verotoxigenic Escherichia coli (VTEC) from Animal Feces for Screening VTEC-shedders

  • Kobayashi, Y. (Graduate School of Agriculture, Hokkaido University) ;
  • Sato, M. (Faculty of Bioresources, Mie University) ;
  • Taguchi, H. (Faculty of Bioresources, Mie University) ;
  • Koike, S. (Graduate School of Agriculture, Hokkaido University) ;
  • Nakatsuji, H. (Graduate School of Agriculture, Hokkaido University) ;
  • Tanaka, K. (Graduate School of Agriculture, Hokkaido University)
  • 투고 : 2003.02.25
  • 심사 : 2003.12.23
  • 발행 : 2004.03.01
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초록

Seventy-six animals including cattle, sheep, horses, 6 species of zoo animals were employed for collection of fresh feces in order to detect verotoxigenic Esherichia coli (VTEC) by safe, quick and sensitive PCR-based molecular methods. Bacterial cell disruption with bead-beating followed by bacterial DNA purification with hydroxyapatide chromatography and gel filtration allowed DNA preparation from animal feces with high recovery and purity. A mountain goat was firstly shown by PCR and sequencing to shed verotoxin 2 gene (vt2) that was used to generate vt2 probe and second primer set for nested PCR to attempt more sensitive detection. Most sensitive nested PCR revealed that 45% of tested cattle and 47% of tested zoo animals were VTEC-positive, while least sensitive normal PCR detected VTEC from none of these animals except a mountain goat. Moderately sensitive detection by PCR in combination with hybridization suggested that the VTEC density varied between the VTEC-positive cattle.

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참고문헌

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피인용 문헌

  1. Year-round Monitoring of Verotoxin-producing Escherichia coli from Feces of Dairy Cattle vol.20, pp.5, 2004, https://doi.org/10.5713/ajas.2007.789