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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Antioxidant Activity of NADH and Its Analogue - An In Vitro Study

  • Olek, Robert Antoni (Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport) ;
  • Ziolkowski, Wieslaw (Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport) ;
  • Kaczor, Jan Jacek (Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport) ;
  • Greci, Lucedio (Dipartimento di Scienze dei Materiali e della Terra, via Brecce Bianche, University of Ancona) ;
  • Popinigis, Jerzy (Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport) ;
  • Antosiewicz, Jedrzej (Department of Bioenergetics, Jedrzej Sniadecki Academy, School of Physical Education and Sport)
  • Published : 2004.07.31
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Abstract

The antioxidant activities of NADH and of its analogue, 1,4-dihydro-2,6-dimethyl-3,5-dicarbethoxy-pyridine ($PyH_2$), were evaluated in vitro. NADH was found to be oxidized by the peroxyl radical derived from 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH) decomposition, in a pH-dependent manner. Both NADH and $PyH_2$ inhibited the peroxidation of egg yolk lecithin (EYL) liposomes, although $PyH_2$ was more effective than NADH when 2,2'-azobis-4-methoxy-2,4-dimethyl-valeronitrile (methoxy-AMVN) was employed to induce EYL liposome peroxidation. The antioxidant activities of NADH and $PyH_2$ were also evaluated by measuring their influences on 1,3-diphenylisobenzofuran (DPBF) fluorescence decay in the presence of peroxyl radicals. NADH and $PyH_2$ were much more effective at inhibiting DPBF quenching in Triton X-100 micelles than in liposomes. These results indicate that NADH can inhibit lipid peroxidation despite being hydrophilic. Nevertheless, membrane penetration is an important factor and limits its antioxidant activity.

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References

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