Inhibitory Effect of Disosium Cromoglycate and Ketotifen on Human Seminal Plasma-Induced Mast Cell Activation

Disodium Kromoglycate와 Ketotifen의 사람정장 유도 비만세포 활성화 억제작용

  • Chai, Ok Hee (Department of Anatomy and Research Center for Allergic Immune Disease, Chonbuk National University Medical School)
  • 채옥희 (전북대학교 의과대학 해부학교실 및 알레르기성 면역질환 연구센터)
  • Published : 2004.09.30

Abstract

Background: Human seminal plasma (HSP)-induced hypersensitivity is one of the serious complications with sexual intercourse. The clinical manifestations of HSP-induced hypersensitivity may be related to the release of vasoactive mediators from mast cell induced by HSP. It has recently been reported that HSP modulates immune systems and induces mast cell degranulation and histamine release from rat peritoneal mast cells (RPMC). Ketotifen and disodium cromoglycate (DSCG), anti-asthmatic and anti-allergic drugs, have a role of mast cell stabilization and inhibit mast cell-induced leukocyte rolling and adhesion. But the inhibitory agents of HSP-induced mast cell activation are unknown. This study was performed to investigate the effects of DSCG and ketotifen on the HSP-induced mast cell activation. Methods: For this, influences of DSCG and ketotifen on the human seminal plasma-induced degranulation, histamine release and morphological changes of RPMC were observed. Results: The mast cell degranulation and histamine release of RPMC by HSP were induced in a dose-dependent fashion. The HSP-induced cytomorphological changes such as swelling, intracellular vacoules, and interrupted cell boundary were significantly inhibited by pretreatment with DSCG or ketotifen. DSCG and Ketotifen inhibited the HSP-induced degranulation and histamine release from RPMC. Conclusion: From the above results, it is suggested that DSCG and ketotifen have a inhibitory effect of the HSP-induced mast cell activation. DSCG and ketotifen may be used for treatment of HSP-induced hypersensitivity.

Keywords

Acknowledgement

Supported by : 보건복지부 보건의료기술진흥사업

References

  1. Bangard C, Rosener I, Merk HF, Baron JM: Type I sensitization to seminal fluid. Urticaria and angioedema. Hautaezt 55;79-81, 2004
  2. Tomitaka A, Suzuki K, Akamatsu H, Matsunaga K: Anaphylaxis to human seminal plasma. Allergy 57;1081-1082, 2002 https://doi.org/10.1034/j.1398-9995.2002.23836_2.x
  3. Shah A, Panjabi C: Human seminal plasma allergy: a review of a rare phenomenon. Clin Exp Allergy 34;827-838, 2004 https://doi.org/10.1111/j.1365-2222.2004.01962.x
  4. Dijkstra AT, Bruijnzeel-Koomen CA: Allergy to human semen. Ned Tijdschr Geneeskd 141;690-692, 1997
  5. 송창호, 한의혁, 조의식, 이창현, 최종림, 이정용, 이무삼: 시험관내 실험에서 사람정장에 의한 흰쥐 복강비만세포 탈과립 및 히스타민 유리. 대한면역학회지 17;81-88, 1995
  6. 송창호, 전성은, 한의혁: 정장이 비만세포 활성화에 미치는 시험관 및 생체실험 I: 정장이 피하반응과 피부 비만세포탈과립에 미치는 영향. 대한체질인류학회지 10;283-291, 1997
  7. Daniele RP: Asthma In: Wyngaarden JB, Smith LH eds.: Cecil textbook of medicine. p403-410, WB Sanders Company, 1989
  8. Cochrane DE, Douglas WW: Calcium-induced extrusion of secretory granule (exocytosis) in the mast cells exposed to 48-80 or the ionophores A-23187 and X-537A. Proc Natl Acad Sci USA 71;408-412, 1974 https://doi.org/10.1073/pnas.71.2.408
  9. Hachisuka H, Kusuhara M, Okubo HK, Sasai Y: Purification of rat peritoneal mast ells with percoll density centrifugation. Arch Dermatol Res 280;358-362, 1988 https://doi.org/10.1007/BF00426614
  10. Harvima RJ, Harvima IT, Fraki JE: Optimization of histamine radioenzyme assay with purifiex histamine-N-methyl transferase. Clinica Chimica Acta 171;247-256, 1988 https://doi.org/10.1016/0009-8981(88)90150-7
  11. James K, Hargreave TB: Immunosuppression by seminal plasma and its possible chinical significance. Immunol Today 5; 357-363, 1984 https://doi.org/10.1016/0167-5699(84)90079-3
  12. Tarter TH, Cunningham-Rundles S, Koids SS: Suppression of natural killer cell activity by human seminal plasma in vitro: identification of 19-OH-PGE as the suppressor factor. J Immunol 136;2862-2867, 1986
  13. Lee HK, Ha TY: Human seminal plasma suppresses delayedtype hypersensitivity responses to intravaginally deposited sheep red blood cells and sperm: seperation of immunosuppressive factors. Int Arch Allergy Appl Immunol 88;412-419, 1989 https://doi.org/10.1159/000234726
  14. Specken JLH: Fen Merkwardig geval van allergi in de gynaedogie. Ned Tjidschr verlosrd gynaecol 58;314-318, 1958.
  15. Poskitt BL, Wojnarowska FT, Shaw S: Semen contact urticaria. J R Soc Med 88;108-109, 1995
  16. Knit B, Degreef H, Dooms-Goossens A: Combined allergy to human seminal plasma and latex: case report and review of the literature. Contact Dermatitis 30;7-11, 1994 https://doi.org/10.1111/j.1600-0536.1994.tb00720.x
  17. Bernstein JA, Jerd ZA, Berstein DI, Korbee L, Berstein IL: Evaluation and treatment of localized vaginal immunoglobulin E-meidated hypersensitivity to human seminal plasma. Obstet Gynecol 82;667-673, 1993 https://doi.org/10.1097/00006250-199310000-00043
  18. Lee HK, Lee HH, Park YH, Lee JH, Ha TY: Regulation of human B cell proliferation and differntiation by seminal plasma. Clin Exp Immunol 85;174-179, 1991 https://doi.org/10.1111/j.1365-2249.1991.tb05700.x
  19. Veselsky L, Dostal J, Kraus M, Peknicova J, Holan V, Zajicova A, Jonakova V, Zelezna B: Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction. Anim Reprod Sci 15;111-23, 2002
  20. Gaboury JP, Johnston B, Niu XF, Kubes P: Mechanisms underlying acute mast cell-induced leukocyte rolling and adhesion in vivo. J Immunol 154;804-813, 1995
  21. Platts-Mills T: Hypersensitivity In:Roitt I, Brostoff J, Male D eds.: Immunology, p330-333, Mosby, 2001