Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Production and diagnostic applications of monoclonal antibodies against porcine circovirus

돼지 써코바이러스에 대한 단크론항체 생산 및 진단적 응용

  • Kim, Kyung-Mi (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University) ;
  • Jeong, Ji-Hye (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University) ;
  • Min, Hong-Ki (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Seung-Chul (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University) ;
  • Roh, In-Soon (National Veterinary Research and Quarantine Service) ;
  • Kang, Shien-Young (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
  • 김경미 (충북대학교 수의과대학/동물의학연구소) ;
  • 정지혜 (충북대학교 수의과대학/동물의학연구소) ;
  • 민홍기 (충북대학교 수의과대학/동물의학연구소) ;
  • 이승철 (충북대학교 수의과대학/동물의학연구소) ;
  • 노인순 (국립수의과학검역원) ;
  • 강신영 (충북대학교 수의과대학/동물의학연구소)
  • Accepted : 2004.05.18
  • Published : 2004.06.30
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Abstract

Porcine circovirus type 2 (PCV-2) has been associated with various disease in pigs worldwide including postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). In this study, monoclonal antibodies (MAbs) against PCV were produced, characterized and applications of MAbs as diagnostic reagents were described. Spleen or lymph node cells from BALB/c mouse immunized respectively with PCV-1, PCV-2 or expressed PCV-2/ORF2 proteins in baculovirus were fused with SP2/0 myeloma cells using polyethylene glycol (PEG) and hybridoma cells producing PCV-1 or PCV-2-specific antibody were screened by an indirect immunofluorescence (IIF) test. A total of fifteen MAbs were produced against PCV. Six MAbs were PCV-1-specific and nine were PCV-2-specific. All PCV-1-specific MAbs reacted with only PCV-1 and all PCV-2-specific MAbs were reactive with only PCV-2 by IIF test. None of the MAbs was reactive with porcine reproductive and respiratory syndrome virus (PRRSV), porcine parvovirus (PPV), porcine rotavirus (PRV), and transmissible gastroenteritis virus (TGEV). Some PCV-2-specific MAbs recognized the PCV-2 infected porcine tissues by IIF or immunohistochemistry (IHC) assay. From this experiment, it was confirmed that MAbs produced in this study were PCV-specific and could be used as reliable diagnostic reagents for PCV-1/PCV-2 detection and differentiation.

Keywords

References

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