Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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A Comparison on Polymorphism of Beta-lactoglobulin Gene in Bos indicus, Bos taurus and Indicine×Taurine Crossbred Cattle

  • Badola, S. (Animal Genetics Division, Indian Veterinary Research Institute) ;
  • Bhattacharya, T.K. (Animal Genetics Division, Indian Veterinary Research Institute) ;
  • Biswas, T.K. (Animal Genetics Division, Indian Veterinary Research Institute) ;
  • Shivakumar, B.M. (Animal Genetics Division, Indian Veterinary Research Institute) ;
  • Kumar, Pushpendra (Animal Genetics Division, Indian Veterinary Research Institute) ;
  • Sharma, Arjava (Animal Genetics Division, Indian Veterinary Research Institute)
  • Received : 2003.07.15
  • Accepted : 2004.01.06
  • Published : 2004.06.01
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Abstract

The study was carried out on eight Bos indicus cattle breeds namely, Sahiwal, Tharparkar, Nimari, Khilari, Deoni, Amritmahal, Hariana and Hilly cattle; two Bos taurus cattle breeds namely, Jersey and Holstein Friesian and Indicine${\times}$Taurine crossbred cattle to find out the polymorphic pattern of beta-lactoglobulin gene. The polymorphism at beta-lactoglobulin gene was detected by conducting PCR-RFLP studies on 398 bp fragment spanning over 104 bases of exon IV and 294 bases of intron IV. Two alleles A and B and three genotypes AA, AB and BB were observed in all the cattle breeds. The frequency of B allele was comparatively higher than that of A allele. The allelic frequency of A varied from 0.20 to 0.30 in Bos indicus cattle breeds and 0.19 to 0.34 in Bos taurus breeds while in crossbred cattle the frequency was estimated as 0.21. The weighted frequency of A allele was highest in Indian cattle and lowest in crossbred cattle while the frequency in taurine cattle was found to be in between indicus and crossbred cattle. The non-significant differences of allelic frequency amongst Bos indicus, Bos taurus and crossbred cattle was observed. The effect of genotype on fat percentage was also found to be non-significant in cattle.

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References

  1. Alexander, L. J., G. Hayes, W. Bawden, A. F. Stewart and A. G. Mackinlay. 1993. Complete nucleotide sequence of the bovine beta-lactoglobulin gene. Anim. Biotechnol. 4:1.
  2. Badola, S., T. K. Bhattacharya, T. K. Biswas, P. Kumar and A. Sharma. 2003. Association of beta-lactoglobulin polymorphism with milk production traits in cattle. Asian-Aust. J. Anim. Sci. 16:1560-1564.
  3. Biswas, T. K., T. K. Bhattacharya, A. D. Narayan, S. Badola, Pushpendra Kumar and A. Sharma. 2003. Growth hormone gene polymorphism and its effect on birth weight in cattle and buffalo. Asian-Aust. J. Anim. Sci. 16:494-497.
  4. Chung, E. R., W. T. Kim and C. S. Lee. 1998. DNA polymorphism of k-casein, beta-1 actoglobulin, growth hormone and prolactin genes in Korean cattle. Asian-Aust. J. Anim. Sci. 11:422-427.
  5. Falconer, D. S. 1998. Introduction to Quantitative Genetics. Ed 4th, Addison Wesley Longman Ltd. Essex, England.
  6. Kim, J. H., H. D. Shin, S. W. Han, B. C. Sang and Y. S. Won. 1997. Polymorphism of kappa-casein and beta-lactoglobulin genes using the polymerase chain reaction in Hanwoo (Korean native) cattle. Kor. J. Anim. Sci. 39:481-488.
  7. Lien, S., S. Rogne, M. J. Brovold and P. Alestrom. 1990. A method of isolation of DNA from frozen (A.I.) bull semen. J. Anim. Breed.Genet. 107:74.
  8. Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A laboratory Manual. Ed 2nd, Vol 3. Cold Spring Harbour Laboratory Press, New York.
  9. Medrano, J. F. and E. Aguilar-Cordova. 1990. Polymerase chain reaction amplification of bovine beta-lactoglobulin genomic sequences and identification of genetic variants by restriction fragment length polymorphism analysis. Anim. Biotechnol. 1:73.
  10. Nguyen, T. C., L. Morera, D. Llanes and P. Leger. 1992. Sheep blood polymorphism and genetic divergence between French Rambouillet and Spanish Merino: Role of genetic drift. Anim. Genet. 23:325-332.
  11. Schlee, P., O. Rottmann and L. Alderson. 1992. Sheep betalactoglobulin determination of alleles A and B by PCR and RFLP analysis using plucked hair as a source of DNA. Genet. Conserv. Livest. 2:243-246.
  12. Snedecor, G. W. and W. G. Cochran. 1967. Statistical Methods. 6th edn. Oxford & IBH Publishing Co. India.
  13. Wilkins, R. J. and Y. M. Kuys. 1992. Rapid beta-lactoglobulin genotyping of cattle using polymerase chain reaction. Anim. Genet. 23:175-178.

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