DOI QR코드

DOI QR Code

Deodorization of Pig Feces by Fungal Application

  • Kim, T.I. (National Livestock Research Institute, Rural Development Administration) ;
  • Ham, J.S. (National Livestock Research Institute, Rural Development Administration) ;
  • Yang, C.B. (National Livestock Research Institute, Rural Development Administration) ;
  • Kim, M.K. (School of Agricultural Biotechnology Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
  • Received : 2003.12.19
  • Accepted : 2004.05.15
  • Published : 2004.09.01

Abstract

This study was carried out to screen yeasts effective in reducing odor from pig feces. Three isolates from soil and compost sources were selected to treat pig feces. On the basis of morphological and biochemical characteristics, one isolate from compost was Candida rugosa, and two isolates from soil were Candida rugosa and Candida maris. These isolates showed deodorizing activity by reducing the concentration of ${NH}_3$ and R-${NH}_2$. Volatile fatty acids (VFA) are the specific malodorous compounds of pig feces, and the Candida maris from soil showed a 100% reduction of butyric, iso-butyric, and iso-valeric acid in 10% pig slurry medium. However, the Candida rugosa from compost showed a 100% reduction of butyric and iso-butyric acid while that from soil showed a 100% reduction of propionic, butyric acid and iso-valeric acid in the medium. Also, these yeasts were effective in reducing $NH_{4}-N$, soluble-N, and biological oxygen demand (BOD).

Keywords

References

  1. AOAC. 1980. Official Method of Analysis, 13th Ed. Association of Official Analytical Chemists, Washington DC.
  2. Babyish, H. and G. Stickly. 1978. Influence of pH on inhibition of bacteria, fungi, coliphages by bisulfate and sulfite. Environ. Res. 15:405-417.
  3. Barnett, J. A., R. W. Payne and D. Yarrow. 1983. Yeasts: Characteristics and Identification. Cambridge University Press, London.
  4. Danaka, O. 1976. Livestock manure treatments by using fungi. Fermentation Engineering 54:333-339.
  5. Danaka, O. 1978. Livestock manure treatments by using Actinomycetes. Fermentation Engineering 56:134-140.
  6. Davise, H. L. 1995. Medically Important Fungi: A Guide to Identification. 3rd Ed. ASM press, Washington, DC.
  7. Dindal, D. L. 1990. Soil sampling and methods of analysis. Soil Biology Guide. Wiley Interscience.
  8. Doring, T. A. 1977. Measurement of odor intensity in farming situations. Agric. Environ. 3:109-120. https://doi.org/10.1016/0304-1131(77)90002-9
  9. Greenberg, A. E. 1992. Standard Methods for the Examination of Water and Wastewater. 18th Ed. American Public Health Association Inc., New York.
  10. Hamano, T., Y. Oka, O. Takada and T. Asano. 1972. Test of malodor composition in the feces of domestic animals. Bull. Hyogo Prefect. Exp. Stan. Anim. Husbandry 9:140-145.
  11. Higaki, S. 1970. Treatment of excrements of domestic animals. Anim. Husbandry 30:119-124.
  12. Kazunari, U., H. Kenta, M. Kohji, K. Yoichi and T. Susumu. 2003. Isolation of Bacillus sp. as a volatile sulfur-degrading bacterium and its application to reduce the fecal odor of pig. Asian-Aust. J. Anim. Sci. 16(12):1795-1798.
  13. Kreger-Van Rij, N. J. W. 1984. The Yeasts (a taxonomic study). 3rd Ed. Elsevier Science Publishers, Amsterdam.
  14. Metcalfe, L. D. and A. A. Schmitz. 1961. The rapid preparation of fatty acids esters for gas chromatographic analysis. Anal. Chem. 33:277-284.
  15. Oho, D. 1991. Deodorization by a microbial method. In research data from the society of livestock manure treatment. Livestock Experimental Station. Japan. pp. 45-52.
  16. Oho, D. 1994. Microbial use of order prevention. The Chemistry of Organisms 32(1):11-12.
  17. Ohta, Y. and M. Ikeda. 1978. Deodorization of pig feces by actinomycetes. Appl. Environ. Microbiol. 36(3):487-491.
  18. Ohta, Y. and Y. Kuwada. 1988. Rapid deodorization of cattle feces by microorganisms. Biol. Wastes. 24:227-240.
  19. Ohta, Y. and H. Sato. 1985. An artificial medium for deodorant microorganism, Agric. Biol. Chem. 49(4):1195-1196.
  20. Olga, F. 1986. Moulds and filamentous fungi in technical microbiology. Progress in Industrial Microbiology Vol. 22. Elsevier sicience Ltd. USA.
  21. Rural Development Association. 1988. Analysis for Soil Chemistry. pp. 243-295.
  22. Samarasinghe, K., T. Shanmuganathan, K. F. S. T. Silva and C. Wenk. 2004. Influence of supplemental enzymes, yeast culture and effective micro-organism culture on gut micro-flora and nutrient digestion at different parts of the rabbit digestive tract. Asian-Aust. J. Anim. Sci. 17(6):830-835. https://doi.org/10.5713/ajas.2004.830
  23. Tanaka, Y., S. Hayashida and M. Hongo. 1977. Treatment of hen feces with fungi. Hakko Kogaku Kaishi 55:134-140.
  24. Yun, S. I. and Y. Ohta. 1997. Some physiological properties of microorganism capable of deodorizing from animal feces. Bio. Technol. 60:21-26.
  25. Yun, S. Y. and S. K. Lee. 1992. Effects of physico-chemical and microbiological inhibitors for odour gas evolution in the fermentation of livestock feces. J. Kor. Soc. Soil Sci. Fert. 25(1):62-69.

Cited by

  1. Study on the reduction of livestock malodor using microbial agents- focusing on swine facilities vol.17, pp.2, 2018, https://doi.org/10.15250/joie.2018.17.2.85
  2. Deodorization study of the swine manure with two yeast strains vol.18, pp.1, 2004, https://doi.org/10.1007/s12257-012-0313-x