Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지

Inhibition of GLUT-1 Expressed in Xenopus laevis Oocytes by Acetoxyscirpendiol of Paecilomyces tenuipes

  • Lee, Dong-Hee (Department of Life Science, The University of Seoul) ;
  • Kim, Ha-Won (Department of Life Science, The University of Seoul)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Paecilomyces tenuipes, a caterpillar fungus, contains many health-promoting ingredients. Recent reports indicate that consumption of P. tenuipes helps reducing blood sugar content for diabetes. Mechanism for reduction in the circulatory sugar content, however, still remains least understood. Methanolic extraction of P. tenuipes (MPT) was prepared and acetoxyscirpendiol (ASD) was subsequently purified limn MPT. Glucose transporter-1 (GLUT-1) was expressed in the Xenopus oocytes and the effect of MPT or ASD on the expressed GLUT-1 was analyzed according to the uptake of 2-dideoxy-D-glucose (2-DOG). MPT was shown to inhibit GLUT-1 activity significant1y compared to the non-treated control. In the presence of ASD and its derivatives, GLUT-1 activity was greatly inhibited in a dose-dependent manner. Among ASD and its derivatives, AS-1 showed most significant inhibition. Taken together, these results strongly indicate that ASD in P. tenuipes may serve as a functional substance in lowering blood sugar in the circulatory system. ASD and its derivatives can be utilized as inhibitors of GLUT-1.

Keywords

References

  1. Baldwin, S. A. (1993). Mammalian passive glucose transporters: members of an ubiquitous family of active and passive transport proteins. Biochim. Biophys. Acta 1154, 17-49 https://doi.org/10.1016/0304-4157(93)90015-G
  2. Bell, G. I., Burant, C.F., Takeda, J., and Gould, G.W. (1993). Structure and function of mammalian facilitative sugar transporters. J. BioI. Chem. 268, 19161-19164
  3. Bell, G.I., Kayano, T., and Buse, J.B., (1990). Molecular biology of mammalian glucose transporters. Diabetes Care 13, 198-208 https://doi.org/10.2337/diacare.13.3.198
  4. Doege, H., Bocianski, A., Joost, H.G., and Schurmann, A. (2000). Activity and genomic organization of human glucose transporter 9 (GLUT9), a novel member of the family of sugartransport facilitators predominantly expressed in brain and leukocytes. Biochem. J. 350, 771-776 https://doi.org/10.1042/0264-6021:3500771
  5. Due, A. D., Qu, Z. C., and Thomas, J. M. (1995). Role of the Cterminal tail of the GLUT-1 glucose transporter in its expression and function in Xenopus laevis oocytes. Biochemistry 34, 5462-5471 https://doi.org/10.1021/bi00016a017
  6. Jones, K. (1997). Cordyceps, Tonic Food of Ancient China, Sylvan Press, Seattle, Washington, pp. 52-61
  7. Kikuchi, H., Miyagawa, Y, Sahashi, Y., Inatomi, S., Haganuma, A., Nakahata, N., and Oshima, Y. (2004). Novel Spirocyclic Trichothecanes, Spirotenuipesine A and B, Isolated from Entomopathogenic Fungus, Paecilomyces tenuipes. J. Org. Chem. 69(2), 352-356 https://doi.org/10.1021/jo035137x
  8. Kirwan, J. P, and del Aguila, L. F. (2003). Insulin signaling, exercise and cellular integrity. Biochem. Soc. Trans. 31,1281-1285 https://doi.org/10.1042/BST0311281
  9. Lee, D.H. (1998). Characterization of 27K zein as a transmembrane protein. J. Biochem. Mol. Biol. 31(2), 196-200
  10. Lee, D.H., Selester, B., and Pedersen, K. (1995). Free movement of 27K zein in the endoplasmic reticulum. Protein Eng. 9, 91-96
  11. Murata, H., Hruz, P.W, and Mueckler, M. (2002). Indinavir inhibits the glucose transporter isoform Glut4 at physiologic concentrations. AIDS 12, 925-926
  12. Nam, K. S., Jo, Y S., Kim, Y. H., Hyun, J. W, and Kim, H.W. (2001). Cytotoxic activities of acetoxyscirpenediol and ergosterol peroxide from Paecilomyces tenuipes. Life Sci. 69(2), 229-237 https://doi.org/10.1016/S0024-3205(01)01125-0
  13. Rumsey, S. C., Daruwala, R., A1-Hasani, H., Zamowski, M. J., Simpson, 1. A., and Levine, M. (2003). Dehydroascorbic acid transport by GLUT4 in Xenopus oocytes and isolated rat adipocytes. J. BioI. Chem. 275, 28246-28253