Effect of Erythromycin on Basal and Stimulated Mucin Release from Cultured Hamster Tracheal Surface Epthelial Cells

  • Park, Ji-Sun (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Seok, Jeong-Ho (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Hur, Gang-Min (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Jae-Heun (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Park, Kyeung-A (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
  • Published : 2004.06.01

Abstract

In the present study, we investigated whether lipopolysaccharide induce mucin release and erythro-mycin affect basal and adenosine triphosphate-induced (stimulated mucin release, from airway goblet cells. Confluent primary hamster tracheal surface epithelial cells were metabolically radiolabeled and chased for 30 min or 24 hr in the presence of varying concentrations of lipopolysaccharide or erythromycin to assess the effects on $^3H$-mucin release. The results were as follows : 1) Lipopolysaccharide failed to induce mucin release, 2) Erythromycin showed no effect on both basal and stimulated mucin release during 30 min of 24 hr treatment period. We conclude that lipopolysaccharide and erythromycin can not affect mucin release by direct acting on airway mucin-secreting cells.

Keywords

References

  1. Ellis, E.F. (1985). Asthma in childhood. J. Allergy Clin. Immunolo 72 (suppl), 526-539
  2. Goswami, S.K., Kivity, S. and Marom, Z. (1990). Erythromycin inhibits respiratory glycoconjugate secretion from human airways in vitro. Am. Rev. Respir. Dis. 141, 72-78 https://doi.org/10.1164/ajrccm/141.1.72
  3. Gray, T., Nettesheim, P., Basbaum, C. and Koo, J. (2001). Regulation of mucin gene expression in human tracheobronchial epithelial cells by thyroid hormone. Biochem. J. 353, 727-734 https://doi.org/10.1042/0264-6021:3530727
  4. Kim, K.C., McCracken, K., Lee, B.C., Shin, C.Y., Jo, M.-J., Lee, C.J. and Ko, K.H. (1997). Airway goblet cell mucin: its structure and regulation of secretion. Eur. Respir. J. 11, 2644-2649
  5. Kim, K.C., Rearick, J.I., Nettesheim, P., and Jetten, A.M. (1985). Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J. Biol. Chem. 260, 4021-4027
  6. Kim, K.C., Wasano, K., Niles, R.M., Schuster, J.E., Stone, P.J. and Brody, J.S. (1987). Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Pmc. Natl. Acad. Sci. USA. 84, 9304-9308 https://doi.org/10.1073/pnas.84.24.9304
  7. Ko, K.H., Lee, C.J., Shin, C.Y., Jo, M.-J. and Kim, K.C. (1999). Inhibition of mucin release from airway goblet cells by polycationic peptides. Am. J. Physiol. 277(21), L811-L815
  8. Kohri, K., Tamaoki, J., Kondo, M., Aoshiba, K, Tagaya, E. and Nagai, A. (2002). Macrolide antibiotics inhibit nitric oxide generation by rat pulmonary alveolar macrophages. Eur. Respir. J. 15(1), 62-67 https://doi.org/10.1183/09031936.00.15106200
  9. Nelson, S., Summer, W.R., Terry, P.B., Warr, G.A. and Jakab, G.J. (1987). Erythromycin-induced suppression of pulmonary antibacterial defenses: a potential mechanism of superinfection in the lung. Am. Rev. Respi.r Dis. 136, 1207-1212 https://doi.org/10.1164/ajrccm/136.5.1207
  10. Palmberg, L., Larsson, B., Malmberg, P. and Larsson, K. (1998). Induction of IL-8 production in human alveolar macrophages and human bronchial epithelial cells in vitro by swine dust. Thorax. 53, 260-264 https://doi.org/10.1136/thx.53.4.260
  11. Richman-Eisenstat, J., Jorens, P.G., Ueki, I. and Nadel, J.A. (1992). Interleukin-8 and neutrophil elastase levels in cystic fibrosis and bronchiectasis sputum. Clin. Res. 40, 237A
  12. Steiger, D., Hotchkiss, J., Bajaj, L., Harkema, J. and Basbaum, C. (1995). Concurrent increase in the storage and release of mucin-like molecules by rat airway epithelial cells in response to bacterial endotoxin. Am. J. Respir. Cell Mol. Biol. 12(3), 307-314 https://doi.org/10.1165/ajrcmb.12.3.7873197
  13. Tagaya, E., Tamaoki, J., Kondo, M. and Nagai, A. (2002). Effect of a short course of clarithromycin therapy on sputum production in patients with chronic airway hypersecretion. Chest. 122(1),213-218 https://doi.org/10.1378/chest.122.1.213
  14. Takeyama, K., Dabbagh, K., Lee, H., Agusti, C., Lausier, J.A., Veki, I.F., Grattan, K.M. and Nadel, J.A. (1999). Epidermal growth factor system regulates mucin production in airways. proc. Natl. Acad. Sci. USA. 6,3081-3086
  15. Tamaoki, J., Isono, K., Sakai, N., Kanemura, T. and Konno, K. (1992). Erythromycin inhibits CI secretion across canine tracheal epithelial cells. Eur. Respir. J. 5, 234-238
  16. Tamaoki, J., Nakata, J., Tagaya, E. and Konno, K. (1996). Effects of roxithromycin and erythromycin on interleukin 8-induced neutrophil recruitment and goblet cell secetion in guinea pig tracheas. Antimicrob. agents Chemother. 40(7), 1726-1728
  17. Tamaoki, J., Sakai, N., Tagaya, E. and Konno, K (1994). Macrolide antibiotics protect against endotoxin-induced vascular leakage and neutrophil in rat trachea. Antimicrob. Agents Chemother. 38(7), 1641-1643 https://doi.org/10.1128/AAC.38.7.1641
  18. Wasano, K., Kim, K.C., Niles, R.M., and Brody, J.S. (1988). Membrane differentiation markers of airway epithelial secretory cells. J. Histochem. Cytochem. 36, 167-178 https://doi.org/10.1177/36.2.3335774