Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Effects of Saururus Chinensis Baill Extracts on Anticancer Activity and Cadmium Induced Cytotoxicity

삼백초 추출물의 항암활성과 카드뮴에 대한 독성억제효과

  • Park Seung-Joung (Dept. of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Yoo Hyun-Ju (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi Hee-Seok (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Seo Byeong-Yun (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yang Seon-Ho (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Yeong-Hun (Dept. of Oriental Medicine, Graduate School, Wonkwang University) ;
  • Jeong Jae-Yeal (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Ki-Nam (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 박승정 (원광대학교 대학원 한의학과) ;
  • 유현주 (원광대학교 한의학전문대학원 제3의학과) ;
  • 최희석 (원광대학교 한의학전문대학원 제3의학과) ;
  • 서병윤 (원광대학교 한의학전문대학원 제3의학과) ;
  • 양선호 (원광대학교 한의학전문대학원 제3의학과) ;
  • 김영훈 (원광대학교 대학원 한의학과) ;
  • 정재열 (원광대학교 한의학전문대학원 제3의학과) ;
  • 이기남 (원광대학교 한의학전문대학원 제3의학과)
  • Published : 2004.12.29
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Abstract

1. The cell viability was determined by MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Saururus chinensis Baill. showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Saururus Chinensis Baill. possessed a potential antitumorous agent. 2. In vitro the antitoxic activity of ethanol extract of Saururus Chinensis Bail on NIH 3T3 fibroblasts was evaluate by the MTT (3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl-2H-tetra캐lium bromide) and SRB (sulforhodamine B protein) assays. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These results suggest that Saururus Chinensis Bail extract retains a potential antitoxic activity. 3. Complexation of Cd (II) ion with ligands such as quercetin has been determined by UV-vis spectrophotometric method in tris buffer solution at various pH. It was found that only 1 : 1 Cd-complex is formed by the interaction between catechol moiety in ring B and cadmium [Cd(II)] in aqueous solution. The spectral parameters for Cd-complex were determined by Beer's law at various pH. It has shown that 1 : 1 Cd-complex has a maximum absorbance and red shift by the alkaline pH.

Keywords

References

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