The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Study of Mylabris Phalerata on Anti-cancer Effects in Some Kinds of Cancer Cells

반모가 수종의 인체 암세포에 미치는 영향

  • Kim, Jin-Sung (Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Yoon, Sang-Hyub (Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Ryu, Bong-Ha (Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Ryu, Ki-Won (Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Myung-Chai (Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 김진성 (경희대학교 한의과대학 비계내과학교실) ;
  • 윤상협 (경희대학교 한의과대학 비계내과학교실) ;
  • 류봉하 (경희대학교 한의과대학 비계내과학교실) ;
  • 류기원 (경희대학교 한의과대학 비계내과학교실) ;
  • 정명채 (경희대학교 한의과대학 비계내과학교실)
  • Published : 2004.06.30
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Abstract

Object : Objective: This study was conducted to investigate the anti-cancer effects of Mylabris phalerata (반모) in some kinds of cancer cells. Materials and Methods: Some kinds of cancer cells lines were treated. We used nine kinds of cancer cell lines, such as stomach cancer cells (Kato), lung cancer cells (Calu-1, NCI-H 1395), urinary bladder cancer cells (HS789T), bone cancer cells (Saos-2), brain cancer cells (SK-N-MC), liver cancer cells (Hep-G2), skin cancer cells (Mo-1) and prostate cancer cells (PC-3) with the water decoction of Mylabris phalerata. The histological changes of all cell lines in the media (RPMI-1640) containing the decoction of Mylabris phalerata were observed and we examined cell death assay by trypan blue exclusion testing was examined. Finally, the change of mitochondrial membrane potential was measurd and the inhibitory effect of Mylabris phalerata on cell increase was examined by analyzing the cell cycle. Results: In histologic change all cancer cell lines showed withdrawn and floating appearance that is typical in cellular impairment. Most of the cell lines showed over 50% death rate after 24 hours in trypan blue exclusion tests. Especially the stomach, urinary bladder. brain and liver cell lines showed over 30% death rate after 12 hours. All cell lines treated with Mylabris phalerata were less stained than the control group and the mitochondrial membrane potential in the Mylabris phalerata treated cell lines was markedly lower than that in the control group. The measurement of DNA quantity in all cell lines showed the disappearance of the peak and the thickened left axis, which suggests that all cellular DNA degraded. Conclusion: Mylabris phalerata had cytotoxicity on various kinds of cancer cell lines and the mechanism of that was the impairment of mitochondria by the breakdown of the mitochondrial cell membrane. We propose that this is in part attributable to the destruction of DNA in cancer cells.

Keywords

References

  1. Curr Gene Ther. v.3 no.5 Integrated, Molecular Engineering Approaches to Develop Prostate Cancer Gene Therapy Wu, L.;Sato, M.D.
  2. Cancer Lett. v.199 no.1 Differentiation-dependent expression of signal transducers and activators of transcription (STATs) might modify responses to growth factors in the cancers of the head and neck Arany, I.;Chen, S.H.;Megyesi, J.K.;Adler-Storthz, K.;Chen, Z.;Rajaraman, S.(et al.)
  3. Oncogene v.22 no.42 Molecular basis of angiogenesis and cancer Tinini, T.;Rossi, F.;Claudio, P.P.
  4. 東醫腫瘍學 崔昇勳
  5. 本草學 全國 韓醫科大學 本草學敎室
  6. 대한한방종양학회지 v.5 no.1 수종한약제의 위암세포에 대한 항암작용 효능 검색 및 약리작용에 관한 분자생물학적 연구 류봉하;김진성;이지향;박재훈;지성길;유진화
  7. 慶熙大學校 韓醫科大學 碩士學位論文 斑모가 TMX 18黑色腫에 미치는 抗癌效果에 對한 硏究 張聖恩;山慈길;蟾수
  8. 大韓韓方內科學會誌 v.22 no.4 四君子湯과 四君子湯加斑모가 胃癌細胞에 미치는 影響 鄭祐榮;柳逢夏;金珍成;尹相協;柳基遠
  9. 慶熙大學校 韓醫科大學 博士學位論文 斑猫 分劃成分의 胃癌細胞에 대한 死滅效果 및 作用機轉에 關한 硏究 朴宰民
  10. 慶熙大學校 韓醫科大學 博士學位論文 斑猫 各 部位에 따른 試驗管 內 人體 胃癌細胞에 對한 效果 林鐘源
  11. Harrison's 내과학(II) 해리슨 번역 편찬위원회
  12. 腫瘤臨證備要 李岩
  13. 腫瘤臨證備要 李岩
  14. 종양학 서울대학교 의과대학
  15. 大韓東醫病理學會誌 v.1 抗癌劑 및 放射線 副作用에 대한 韓方治療 金東熙;金聖勳
  16. 慶熙大大學院 碩士學位論文 消癌去痰益氣湯의 抗癌效果에 대한 硏究 신병철;이형구;김병우;유기원;유봉하;윤상협
  17. 大韓韓方腫瘍學會誌 v.4 no.1 六味地黃湯이 生理活性指標와 淋巴球細胞數에 미치는 影響 金榮權;柳逢夏;朴東源;柳基遠
  18. 大韓韓方腫瘍學會誌 v.6 no.1 香砂平胃散이 抗癌 및 免疫調節作用에 미치는 影響 하지용;조영호;이지숙
  19. 大韓韓方腫瘍學會誌 v.5 no.1 血府逐瘀湯이 癌轉移 抑制에 미치는 影響 李眞華;沈範相;安圭錫;崔昇勳
  20. 大韓韓方腫瘍學會誌 v.5 no.1 消積白朮散이 Bleomycin의 副作用減少와 抗癌效果에 미치는 影響 金泰運;孫彰奎;趙鍾寬
  21. 本草學 李尙仁
  22. 大韓韓方內科學會誌 v.15 no.2 斑猫에 관한 문헌적 고찰 박미형;조종관
  23. 本草求眞 黃宮繡
  24. 新編中藥大辭典(下) 新文豊出版公司(編)
  25. The journal of Histochemistry & Cytochemistry v.47 no.9 Cytochemial methods for the dectection of apoptosis Mark, C.W.
  26. 大韓腫瘍學會誌 v.32 no.1 방사선 조사에 의한 HL-60 세포주의 Apoptosis 신호전달기전 안성자;박래길;이상록;정웅기;나병식;남택근(등)
  27. Am J Respir Cell Mol Biol. v.25 no.6 Reactive oxygen species and cell signaling John, H.
  28. Eur J Biochem. v.264 Mitochondira and cell death: mechanistic aspecs and methodological issues Paolo, B.;Luca, S.;Raffaele, C.;Valeria, P.;Fabio, D.L.
  29. Biochem J. v.342 The mitochondiral permeability transition pore and its role in cell death Martin, C.
  30. 大韓腫瘍學會誌 v.332 no.4 Wild-type p53 유전자가 결여된 암세포에서 cyclic AMP의 세포주기 조절기작에 관한 연구 장정순;강정훈;박병규;황상구