국내산 주요 갈조류 추출물의 항혈액응고 활성

Anticoagulant Activities of Brown Seaweed Extracts in Korea

  • 발행 : 2004.12.31

초록

국내산 주요 갈조류 18종의 추출용매 및 온도에 따른 항혈액응고 활성을 측정한 결과 미역, 다시마, 감태, 대황, 곰피, 톳, 괭생이 모자반의 열수 추출물의 APTT가 190.0초 이상으로 활성이 높았으며, 이 때의 추출온도는 $90^{\circ}C$였다. 감태의 에탄올 불용성분의 항혈액응고 활성은 $500\;{\mu}g/mL$에서 에탄을 가용성 물질에 비하여 2배 이상 증가하였으며, 에탄을 불용성분의 분자량에 따른 항혈액응고 활성은 $100\;{\mu}g/mL$ 농도에서 EKJ-eim 1(100 kDa 이상)이 190초 이상으로 EKJ-eim 2(100-50 kDa) 및 EKJ-eim 3(50-10 kDa)에 비해 좋았으며, 이 분회의 화학적 조성성분은 fucose, xylose, mannose, galactose, glucose, sulfate로 구성되어 있으며, 몰비는 1 : 0.05 : 0.10 : 0.15 : 0.17 : 1.46으로 2 mole의 fucose에 3 mole의 황산기가 결합되어 있는 산성다당임을 확인하였다.

Effects of extraction conditions and molecular fractionation on anticoagulant activities of major brown seaweeds in Korea were investigated. Hot water extracts of C. costata, U. pinnatifida (Sporophyte), L. japonica, K. crassifolia, E. stolonifera, E. bicyclis, S. horneri, and E. kurome increaced activated partial thromboplastin time (APTT) over 190 seconds, which may be related to intrinsic pathway of blood coagulation. Hot water extract of E. Kurome (EKJ) was further fractionated by ethanol precipitation. EKJ-eim, ethanol-insoluble material of EKJ, showed higher anticoagulant activity than EKJ. EKJ-eim was further fractioned with ultrafiltration. EKJ-eim 1, (over 100 kDa) fraction showed higher APTT activity than EKJ-eim. A EKJ-eim 1 was sulfated polysaccharide consisting of fucose, xylose, mannose, galactose, glucose and, sulfate at molar ratio of 1 : 0.05 : 0.10 : 0.15 : 0.17 : 1.46. The anticoagulant activity increased as sulfate content and molecular weight increased.

키워드

참고문헌

  1. Ito K, Hori K. Seaweed: chemical composition and potential food uses. Food Rev. Int. 5: 101-144 (1989) https://doi.org/10.1080/87559128909540845
  2. Nishino T, Yokoyama G, Dobashi K, Fujihara M, Nagumo T. Isolation, purification, and characterization of fucose containing sulfated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities. Carbohydr. Res. 186: 119-129 (1989) https://doi.org/10.1016/0008-6215(89)84010-8
  3. Percival E, McDowell RH. Chemistry and Enzymology of Marine Algal Polysaccharides. Academy Press Inc. London, UK (1967)
  4. Richard L, Mueller MD. History of drugs for thrombotic disease. Circulation 89: 432-450 (1994) https://doi.org/10.1161/01.CIR.89.1.432
  5. Jackson CM, Nemerson Y. In Blood Coagulation. Ann. Rev. Biochem. 49: 765-771 (1980) https://doi.org/10.1146/annurev.bi.49.070180.004001
  6. Shim YY, An JH, Cho WD, Chun H, Kim KI, Cho HY, Yang HC. Inhibitory mechanism of blood coagulation and in vivo anticoagulant activities of polysaccharides isolated from Codium fragile. J. Korean Soc. Food Sci. Nutr. 31: 917-923 (2002) https://doi.org/10.3746/jkfn.2002.31.5.917
  7. Jaques LB. Heparins: anionic polyelectroylte drugs. Pharmacol. Exp. Therap. 31: 99-166 (1979)
  8. Kweon MH, Park MK, Ra KS, Sung HC, Yang HC. Screening of anticoagulant polysaccharides from edible plants. Agric. Chem. Biotech. 39:159-164 (1996)
  9. Kim HS, Nam KH, Lee DH. Measurement of PT, APTT, and fibrinogen by automatic coagulation analyzer, sysmex CA-540. J. Clin. Pathol. Qual. Cont. 23: 247-252 (2001)
  10. Dubois M, Gills KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for the determination of sugars and related substances. Anal. Chem. 28: 350-352 (1956) https://doi.org/10.1021/ac60111a017
  11. Lowry OH, Rosebrough NJ, Farr L, Rindall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 256-275 (1951)
  12. Dodgson KS, Price RG. A note on the determination of the ether sulphate content of sulphated polysaccharides. J. Biochem. 84: 106-107 (1962) https://doi.org/10.1042/bj0840106
  13. Blumenkronz N, Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal. Biochem. 54: 484-487 (1973) https://doi.org/10.1016/0003-2697(73)90377-1
  14. Furneaux RH, Miller IJ, Stevenson TT. Agaroids from New Zealand members of the Gracilariaceae (Gracilariales, Rhodophyta)- a novel dimethylated agar. Hydrobiologia 204: 645-650 (1990) https://doi.org/10.1007/BF00040300
  15. Yoon JA, Yu KW, Jun WJ, Cho HY, Son YS, Yang HC. Screening of anticoagulant activity in the extracts of edible seaweeds and optimization of extraction condition. J. Korean Soc. Food Sci. Nutr. 29: 1098-1106 (2000)
  16. Koo JG, Jo KS, Do JR, Woo SJ. Isolation and purification of fucoidans from Laminaria religiosa and Undaria pinnatifida in korea. J. Korean Fish. Soc. 28: 227-236 (1995)
  17. Park MK, Kweon MH, Cho HY, Yang HC. Anticoagulant activity of sulfated polysaccharides isolated from Codium fragile. J. Korean Soc. Agric. Chem Biotech. 42: 140-146 (1999)
  18. Nishino T, Nagumo T. The sulfate-content dependence of the anticoagulant activity of a fucan sulfate from the brown seaweed Ecklonia kurome. Carbohydr. Res. 214: 193-197 (1991) https://doi.org/10.1016/S0008-6215(00)90542-1