Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Identification Characteristics of Irradiated Dried Red Pepper during Storage by Analysis of Thermoluminescence, DNA Comet, and DEFT/APC

Thermoluminescence, DNA Comet 및 DEFT/APC 분석에 의한 방사선처리 건고추의 저장 중 검지 특성

  • Kim, Byeong-Keun (Department of Food Science and Technology, Kyungpook National University) ;
  • Kwon, Joong-Ho (Department of Food Science and Technology, Kyungpook National University)
  • Published : 2004.12.31
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Abstract

Minerals separated from irradiated dried red pepper (whole) at 2.5 kGy or higher showed typical thermoluminescence (TL) glow curves ($TL_1$) at around $150^{\circ}C$, which increased with irradiation dose. The TL ratio ($TL_1/TL_2$) through re-irradiation step at 1 kGy enhanced reliability of TL identification results. DNA comet assay indicated that the intact cell was observed in non-irradiated pepper (seed), while some long tails were found in irradiated ones, showing relationship between irradiation dose and tail length. Log DEPT/APC values increased in proportion to irradiation doses in powdered and whole peppers. Based on overall results, irradiated dried red peppers could be screened using DNA comet assay or log DEFT/APC, and moreover the identification results were verified by TL analysis.

통고추의 열발광 특성(TL)분석에서 2.5 kGy 이상의 조사 시료에서 분리된 미네랄 시료는 $150^{\circ}C$ 부근에서 TL glow curve ($TL_1$)를 나타내었고, 조사선량이 증가할수록 peak intensity가 증가하였다. 재조사(1 kGy) 방법에 의한 TL ratio($TL_1/TL_2$)는 TL 분석의 신뢰도를 높여주었다. DNA comet assay에서 비조사 시료(씨)는 전형적인 intact cell을 나타내었으나 감마선 조사 시료에서는 long tail을 가진 comet을 나타내면서 선량 의존적으로 tail length가 증가되었다. 미생물학적 방법으로써 DEFT/APC값 측정에서는 조사선량에 따라 분말고추와 통고추에서 비교적 높은 상관을 보였다. 이상의 결과에서 DNA comet assay와 DEFT/APC 방법은 방사선 조사 건고추의 screening 방법으로서, TL은 확인방법으로서 적용가능성이 확인되었다.

Keywords

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