콩의 발아 중 이소플라본과 올리고당의 변화

Changes in Isoflavone and Oligosaccharides of Soybeans during Germination

  • Kim, Joo-Sook (Department of Human Life Science, Sejong University) ;
  • Kim, Jong-Goon (Department of Human Life Science, Sejong University) ;
  • Kim, Woo-Jung (Department of Food Science and Technology, Sejong University)
  • 발행 : 2004.04.30

초록

세 가지 품종의 한국산 콩을 발아시키면서 isoflavone과 올리고당, 건물량의 변화를 조사하였다. 실험에 사용된 콩은 명주 나물콩, 태광콩, 다원콩이었으며 콩의 발아는 $20^{\circ}C$의 항온기에서 10시간 수침 후 8일간 발아시켰다. 세 가지 품종 중 isoflavone 함량이 가장 높았던 품종은 명주나물콩(1.288mg/g)이었으며 그 다음은 태광콩(0.671 mg/g), 다원콩(0.661 mg/g)이었다. 총 isoflavone함량은 전반적으로 발아 초기에 증가하였고 그 후는 감소하는 경향을 보였다. 최고값에서의 증가율은 20-50%의 범위였으며 특히 daidzein과 genestein등 aglycone 형태의 증가가 더욱 현저하였다 올리고당인 stachyose와 raffinose의 함량은 발아 중 빠르게 감소하였으나 sucrose의 감소는 비교적 완만하였다. 건물량은 발아 중 서서히 감소하였고 뿌리의 성장은 발아 2일부터 시작하였다.

Three Korean soybean varieties, Dawon, Taekwang, and Myeongiju-namul were investigated for changes in isoflavone and oligosaccharides contents, and dry weight during germination. Soybeans were soaked for 10 hr in water, followed by 8 days germination at $20^{\circ}C$ under dark condition, Highest isoflavone content measured was Myeongju-namul (1.228 mg/g), followed by Taekwang (0.671 mg/g) and Dawon (0.661 mg/g). Total isoflavone content generally increased during initial germination and decreased thereafter. Maximal increase in isoflavone was 20-50%, particularly in aglycone type such as daidzein and genistein. Raffinose and stachyose contents decreased rapidly during germination, while that of sucrose showed relatively slow decrease. Dry weights of soybeans steadly decreased.

키워드

참고문헌

  1. Coward L, Barnes NC, Kenneth DR, Setchell DR, Barnes S. Genistein, daidzein, and their $\beta$ -glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets. J. Agric. Food Chem. 41: 1961-1967 (1993) https://doi.org/10.1021/jf00035a027
  2. Kennedy AR. The evidence for soybean products as cancer preventive agents. J. Nutr. 125: 733-743 (1995)
  3. Kwoon HJ. Bioactive compounds of soybean and their activity in angiogenesis regulation. Korean Soybean Digest. 16: 63-68 (1999)
  4. Kim JS. Current research trends on bioactive function of soybean. Korean Soybean Digest. 13: 17-24 (1996)
  5. Shon HS, Lee YS, Shin HC, Chung HK. Does soybean isoflavone have adverse effects on human? Korean Soybean Digest. 17: 9-19 (2000)
  6. Messina MJ, Persky V, Setchell KD, Barnes S. Soy intake and cancer risk : a review of the in vitro and in vivo data. Nutr. Cancer 21: 113-131 (1994) https://doi.org/10.1080/01635589409514310
  7. Moon BK, Jeon KS, Hwang IK. Isoflavone contents in some varieties of soybean and on processing conditions. Korean J. Soc. Food Sci. 12: 527-534 (1996)
  8. Dwyer JT, Goldin BR, Saul N, Gualtieri L, Barakat S, Adlercreutw H. Tofu and soy drinks contain phytoestrogens. J. Am. Diet Assoc. 94: 743-793 (1994)
  9. Eldridge AC, Kwoler WF. Soybean isoflavones: effect of environment and variety on composition. J. Agric. Food Chem. 31: 394-396 (1983) https://doi.org/10.1021/jf00116a052
  10. Yang CB, Kim ZU. Changes in nitrogen compounds in soybean sprout. J. Korean Agric. Chem. Soc. 23: 7-13 (1980)
  11. Lee SH, Chung DH. Studies on the effects of plant growth regulator on growth and nutrient compositions in soybean sprout. J. Korean Agric. Chem. Soc. 25: 75-82 (1982)
  12. Kim WJ, Smit CJB, Nakayama TOM. The removal of oligosaccharides from soybeans. Lebensm. Wiss. U. Technol. 6: 201-204 (1973)
  13. Wang G, Kuan SS, Francis OJ, Ware GM, Carman AS. A simplified HPLC method for the determination of phytoestrogens in soybean and its proceded products. J. Agric. Food Chem. 38: 185-190 (1990) https://doi.org/10.1021/jf00091a041
  14. Park MH. Purification and separation of soy-oligosaccharides from defatted soybean meal. PhD thesis, Seoul National University, Seoul, Korea (2001)
  15. Kim KS, Chung HK, Sohn HS. Purification of oligosaccharides from soybean using activated charcoal. Food Sci. Biotechnol. 3: 156-159 (1994)
  16. Kim DH, Choi HS, Kim WJ. Comparison study of germination and cooking rate of several soybean varieties. Korean J. Food Sci. Technol. 22: 94-98 (1990)
  17. Lee YH, Jung HO, Rhee CO. Solids loss water uptake during soaking of soybeans. Korean J. Food Sci. Technol. 19: 492-498 (1987)
  18. Wang HJ, Murphy PA. Mass balance study of isoflavones during soybean processing. J. Agric. Food Chem. 44: 2377-2383 (1996) https://doi.org/10.1021/jf950535p
  19. Kim KW, Chun BS. Optimum contersion to the aglycone form using $\beta$ -glucosidase and isoflavone extraction from soybean. Korean J. Biotechnol. Bioeng. 16: 174-178 (2001)
  20. Chang KC, Chang DC, Phatac L. Effect of germination on oligosaccharides and nonstarch polysaccharides in navy and pinto beans. J. Food Sci. 54: 1615-1619 (1989) https://doi.org/10.1111/j.1365-2621.1989.tb05173.x
  21. Cruz R, Batistela JC, Wosiacki G. Microbial $\alpha$-galactosidase for soymilk processing. J. Food. Sci. 46: 1196-1200 (1981) https://doi.org/10.1111/j.1365-2621.1981.tb03022.x