Fermentation Characteristics of Low Salted Kochujang Prepared with Sub-materials

부원료를 첨가한 저식염 고추장의 발효특성 변화

  • Kim, Dong-Han (Department of Food and Nutrition, Mokpo National University) ;
  • Yang, Sung-Eun (Department of Food and Nutrition, Mokpo National University)
  • 김동한 (목포대학교 생활과학부 식품영양학 전공) ;
  • 양성은 (목포대학교 생활과학부 식품영양학 전공)
  • Published : 2004.02.28

Abstract

Effects of sub-materials such as, ethanol, mustard, and chitosan, on enzymatic, microbial and physicochemical characteristics of kochujang were investigated during fermentation. Activity of ${\alpha}$-amylase was low in chitosan-added kochujang, whereas those of ${\beta}$-amylase and pretense did not show any remarkable difference. Viable cells of yeast and bacteria decreased in sub-material-added kochujang during fermentation, with yeast counts decreasing more rapidly in ethanol- and mustard-added kochujang than that with chitosan. Consistency of kochujang decreased during fermentation, with the highest consistency observed in ethanol-added kochujang. Oxidation-reduction potential was low in chitosan-added kochujang. Water activity of all kochujang groups decreased during fermentation with the lowest in ethanol-added kochujang. Hunter L-, a-, and b-values of chitosan-added kochujang were higher than other groups, whereas increase in total color difference of low-salt without sub-material group was lowest pH of kochujang was the highest in ethanol-added kochujang, whereas titratable acidity increased remarkably in chitosan-added group. Total sugar and reducing sugar contents of kochujang were high in ethanol-and mustard-added groups, whereas ethanol production decreased remarkably in mustard-added kochujang. Amino nitrogen content was highest in low-salt without sub-material kochujang during late aging period. Ammonia nitrogen content was lower in mustard-added kochujang. After 12 weeks of fermentation, ethanol-added kochujang was more acceptable than mustard-and chitosan-added groups in taste, color, and overall acceptabilities.

저식염 고추장을 제조하기 위하여 고추장의 소금농도를 9%에서 6%로 줄인 후 알콜 또는 겨자, 키토산을 첨가하여 숙성중의 품질특성을 비교하였다. 고추장의 전분액화효소의 활성은 키토산 첨가구에서 낮았으나 전분당화효소와 protease의 차이는 적었다. 고추장 숙성중의 효모수와 세균수는 알콜과 겨자, 키토산의 첨가로 감소되었으며, 효모수의 감소는 키토산 첨가에 비하여 알콜과 겨자의 첨가 고추장에서 심하였다. 고추장의 점 조성은 숙성중에 감소하나, 알콜 첨가구에서 높았다. 고추장의 산화환원전위는 키토산 첨가구에서 낮았으며 수분활성도의 저하는 알콜 첨가구에서 심하였다. 키토산 첨가 고추장이 숙성후에 L-과 a-, b-값이 높아 밝고 붉은 색이 진했으나, 저식염 무첨가구가 ${\Delta}E$의 변화가 적었다. 고추장의 pH는 알콜 첨가구에서 높았고 키토산의 첨가로 적정산도의 증가가 심하였다. 고추장의 총당과 환원당은 알콜과 겨자 첨가구에서 높았으며, 알콜의 생성은 겨자의 첨가로 현저하게 억제되었다. 고추장의 아미노태 질소는 저식염 무첨가구에서 높았으며 암모니아태 질소의 생성은 겨자 첨가구에서 낮았다. 12주 숙성시킨 고추장의 맛과 색, 종합적인 기호도는 알콜 첨가 고추장이 양호하여 저식염 고추장의 제조는 소금의 일부를 알콜로 대체하여 첨가하는 것이 효과적이었다.

Keywords

References

  1. Lee TS. Studies on the brewing of kochujang (red papper paste) by the addition of yeasts. J. Korean Agric. Chem. Soc. 22: 65-90 (1979)
  2. Cho HO, Kim JG, Lee HJ, Kang JH, Lee TS. Brewing method and composition of traditional kochujang (red pepper paste) in junrabook-do area. J. Korean Agric. Chem. Soc. 24: 21-28 (1981)
  3. Choi JY, Lee TS, Noh BS. Quality characteristics of the kochujang prepared with mixture of meju and koji during fermentation. Korean J. Food Sci. Technol. 32: 125-131 (1998)
  4. Oh HI, Shon SH, Kim JM. Physicochemical properties of kochujang prepared with Aspergillus oryzae, Bacillus licheniformis and Saccharomyces rouxii during fermentation. J. Korean Soc. Food Sci. Nutr. 29: 357-363 (2000)
  5. Lee KS, Kim DH. Trial manufacture of low-salted kochujang (red pepper soybean paste) by the addition of alcohol. Korean J. Food Sci. Technol. 17: 146-154 (1985)
  6. Cho HO, Park SA, Kim JG. Effect of traditional and improved kochujang koji on the quality improvement of traditional kochujang. Korean J. Food Sci. Technol. 13: 319-327 (1981)
  7. Kwan DJ, Jung JW, Kim JH, Park JY, Yoo JY, Koo YJ, Chung KS. Studies on establishment of optimal aging time of Korean traditional kochujang. Agric. Chem. Biotech. 39: 127-133 (1996)
  8. Yeo YK, Kim ZU. Studies on the standardization of the processing conditional of Ko-Choo-Jang(red pepper paste). J. Korean Agric. Chem. Soc. 21: 16-21 (1978)
  9. Kim YS, Cha J, Jung SW, Park EJ, Kim JO. Changes of physicochemical characteristics and development of new quality indices for industry-produced koji kochujang. Korean J. Food Sci. Technol. 26: 453-458 (1994)
  10. Moon TW, Kim ZU. Some chemical physical characteristics and acceptability of kochujang from various starch sources. J. Korean Agric. Chem. Soc. 31: 387-393 (1988)
  11. Shin DH, Kim DH, Choi U, Lim MS, An EY. Physicochemical characteristics of traditional kochujang prepared with various raw materials. Korean J. Food Sci. Technol. 29: 907-912 (1997)
  12. Kim YS. Studies on the changes in physicochemical characteristics and volatile flavor compounds of traditional kochujang during fermentation. PhD thesis, University of King Sejong, Seoul, Korea (1993)
  13. Kim MS, Ahn EY, Ahn YS, Shin DH. Characteristic change of kochujang by heat treatment. Korean J. Food Sci. Technol. 32: 867-874 (2000)
  14. Jung SW, Kim YH, Koo MS, Shin DB. Changes in physicochemical properties of industry-type kochujang during storage. Korean J. Food Sci. Technol. 26: 403-410 (1994)
  15. Kim GT, Hwang YI, Lim SI, Lee DS. Carbon dioxide production and quality changes in Korean fermented soybean paste and hot pepper-soybean paste. J. Korean Soc. Food Sci. Nutr. 29: 807-813 (2000)
  16. Chun MS, Lee TS, Noh BS. Effect of gamma-irradiation on quality of kochujang during storage. Foods Biotechnol. 1: 117-122 (1992)
  17. Lee KY, Kim HS, Lee HG, Han O, Chang UJ. Studies on the prediction of the shelf-life of kochujang through the physicochemical and sensory analyses during storage. J. Korean Soc. Food Sci. Nutr. 26: 588-594 (1997)
  18. Lee KS, Kim DH. Effect of sake cake on the quality of low salted kochujang. Korean J. Food Sci. Technol. 23: 109-115 (1991)
  19. Yamamoto Y, Higashi K, Yoshii H. Inhibitory activity of ethanol on food spoilage bacteria. Nippon Shokuhin Kogyo Gakkaishi 31: 531-535 (1984) https://doi.org/10.3136/nskkk1962.31.8_531
  20. Kim DH, Lee JS. Effect of condiments on the physicochemical characteristics of traditional kochujang during fermentation. Korean J. Food Sci. Technol. 33: 353-360 (2001)
  21. Yun YS, Kim KS, Lee YN. Antibacterial and antifungal effect of chitosan. J. Chitin Chitosan 4: 8-14 (1999)
  22. Shim KH, Seo KI, Kang KS, Moon JS, Kim HC. Antimicrobial substances of distilled components from mustard seed. J. Korean Soc. Food Nutr. 24: 948-955 (1995)
  23. Shin DH, Ahn EY, Kim YS, Oh JA. Fermentation characteristics of kochujang containing horseradish or mustard. Korean J. Food Sci. Technol. 32: 1350-1357 (2000)
  24. Kim DH, Kwon YM. Effect of storage conditions on the microbiological and physicochemical characteristics of traditional kochujang. Korean J. Food. Sci. Technol. 33: 589-595 (2001)
  25. IMT. Official Methods of Miso Analysis. Institute of Miso Technologists, Tokyo, Japan. pp. 1-34 (1968)
  26. Thomas YD, Lulvwes WJ, Kraft AA. A convenient surface plate method for bacteriological examination of poultry. J. Food Sci. 46: 1951-1952 (1981) https://doi.org/10.1111/j.1365-2621.1981.tb04528.x
  27. Difco Laboratories. Difico Manual. 19th ed. Difico, Detroit, MI, USA. pp. 1064-1065 (1965)
  28. Martin EP. Use of acid, rose bengal, and streptomycin in the plate method for estimating soil fungi. Soil Sci. 69: 215-232 (1965)
  29. Fuwa HA. A new method for microdetermination of amylase activity by the use of amylose as the substrate. J. Biochem. 41: 583-588 (1954) https://doi.org/10.1093/oxfordjournals.jbchem.a126476
  30. Anson ML. Estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22: 79-89 (1938) https://doi.org/10.1085/jgp.22.1.79
  31. SAS Institute, Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1992)
  32. Jay JM. Modern Food Microbiology, 6th ed. Kluwer Academic/Plenum Publishers, New York, NY, USA. pp. 45-47 (2000)
  33. Oh JY, Kim YS, Shin DH. Changes in physicochemical characteristics of low-salted kochujang with natural preservatives during fermentation. Korean J. Food Sci. Technol. 34: 835-841 (2002)
  34. Jung YC, Choi WJ, Oh NS, Han MS. Distribution and physiological characteristics of yeasts in traditional and commercial kochujang. Korean J. Food Sci. Technol. 28: 253-259 (1996)