Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Extraction Characteristics and Antioxidative Activity of Schiznadra chinensis Extracts

오미자 추출물의 추출특성 및 항산화 효과

  • Published : 2004.10.31
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Abstract

The present study was conducted to investigate extraction characteristics and antioxidative activity of Schiznadra chinensis extracts. Schiznadra chinensis was extracted by reflux extraction(RE) under different extraction conditions including solvent. The solid yield, turbidity, color value, titratable acidity, free sugar contents, electron donating ability(EDA) and superoxide dismutase(SOD)-like ability of Schiznadra chinensis extracts were determined. The highest solid yield value was obtained with water of 10 fold. No significant difference in turbidity and color value were found among the extracts prepared with various extraction solvents, 75% ethanol, 50% ethanol and water. The highest titratable acidity was obtained with water extracts of Schiznadra chinensis. The free sugar contents of Schiznadra chinensis extracted with water showed the highest value. Schiznadra chinensis extracts with water included higher contents of free sugar compared with those of the other solvent extracts,50% ethanol and 75% ethanol extracts. The total polyphenol compound content of Schiznadra chinensis extracted with 50% ethanol showed the highest value. Schiznadra chinensis extracts with 50% ethanol included higher contents of total polyphenol compound compared with those of the other solvent extracts, water and 75% ethanol extracts. The electron donating ability of extracts were 60.87% in water, 57.24% in 50% ethanol, and 55.61% in 75% ethanol.

오미자 추출액의 추출 특성과 추출액의 항산화성을 검토하여 오미자의 추출 조건을 결정함으로써 오미자 유효성분의 식품 소재화를 위한 기초자료를 얻고자 하였다. 용매와의 비율을 결정하기 위해 8배, 10배, 12배, 14배의 water를 가하여 추출 수율을 비교한 결과 water와의 비율이 10배일 때 추출 수율이 가장 좋은 것으로 나타났다. 탁도는 Water 추출액에서 가장 높았으며, 추출용매의 ethanol 농도가 증가할수록 낮아졌다. 적정산도는 추출용매의 50% ethanol에서 가장 높았으나, 전체적으로 산도가 매우 낮았다. 유리당 함량은 fructose $1.24{\sim}4.42mg%$, glucose $3.65{\sim}4.71mg%$, sucrose $0.89{\sim}1.69mg%$로 glucose의 함량이 가장 높았으며, 당질의 대부분을 glucose와 fructose인 것으로 나타났다. 총 폴리페놀 함량을 나타낸 것으로 50% ethanol 추출액은 0.98%, 75% ethanol 추출액은 0.89%, water 추출액은 0.58%로 나타나 50% ethanol 추출액에서 가장 높은 함량을 나타내었으며. water에 비해 유기용매 추출시 높은 함량을 보였다. 전자공여능은 water 추출액 60.87%, 50% ethanol 추출액 57.24%, 75% ethanol 추출액 55.61%로 나타나 용매별 전자공여능 효과가 큰 차이는 나타나지 않았으며. water 추출액이 다른 용매에 비해 높은 전자공여 효과를 보여 주었다. SOD 유사환성 측정 결과는 50% ethanol 추출액에서 33.65%로 가장 높은 활성을 나타냈었으며, 50% ethanol 추출액이 다른 두 용매 보다 우수한 것으로 나타나 총 폴리페놀 함량과 전자공여능 측정 결과 와 비슷한 경향을 보여주었다.

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References

  1. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA 90: 7915-7922, 1993 https://doi.org/10.1073/pnas.90.17.7915
  2. Choi JS, Jung JH, Lee HJ, Lee JH, and Kang SS. A naphthalene glycoside from Cassia tora. Phytochemistry. 40: 997-999, 1995 https://doi.org/10.1016/0031-9422(95)00318-2
  3. Devy C, and Gautier R. New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase. Biochem. Parmacol. 39: 399-405, 1990 https://doi.org/10.1016/0006-2952(90)90043-K
  4. Kuramoto T. Development and application of food materials from plant extract such as SOD. Upto date Food Processing 27(3): 22-23, 1992
  5. Hae J. DongEuBoGam, NamSanDang, Seoul, pp.140-142, 1990
  6. Takahashi N, Kawada T, Goto T, Yamamoto T, Taimatsu A, Mataui N, Kimura K, Saito M, Hosokawa M, Miyashita K, and Fushiki T. Dual action of isoprenols from herbal medicines on both PPARgamma and PPARaloha in 3T3-L1 adipocytes and HepG2 Hepatocytes. FEBS Lett. 514: 315-322, 2002 https://doi.org/10.1016/S0014-5793(02)02390-6
  7. Kamei R, Kadokura M, Kitagawa Y, Hazeki O, and okigawa s. 2'-benzyloxychalcone derivatives stimulate glucose uptake in 3T3-L1 adipocytes. Life Sci. 73: 2091-2099, 2003 https://doi.org/10.1016/S0024-3205(03)00563-0
  8. Bao TT, Liu GT, Song ZY, Xu GF and Sun RH. Acomparison of the pharmacologic actions of 7 constituents isolated from Fructus schizandrae. J. Chin. Med. 93: 41-47
  9. Members of Pharmacognosy. In hyundDai pharmacognoay, Hak ChangSa, pp. 434-438
  10. Ikeya Y, Taguchi H, Yasioka I, and Kobayashi H. The constituents of Schizandra chinensis B. I. isolation and structure determination of five new lignans, gomisin A, B, C F and G, and the absolute structure of schizandrin, Chem. Pharm. Bull. 27(6): 1383, 1979 https://doi.org/10.1248/cpb.27.1383
  11. Ikeya Y. Taguchi H, Yasioka I, and Kobayashi H. The constituents of Schizandra chinensis B. II. isolation and structure determination of five new lignans, gomisin A, B, C F and G, and the absolute structure of schizandrin, Chem. Pharm. Bull. 26(10): 1383, 1978
  12. Ikeya Y. Taguchi H, Yasioka I. The constituents of Schizandra chinensis B. II. isolation and structure determination of five new lignans, gomisin A, B, C F and G, and the absolute structure of schizandrin, Chem. Pharm. Bull. 27(10): 2536, 1979 https://doi.org/10.1248/cpb.27.2536
  13. Ikeya Y. Taguchi H, Yasioka I, and Kobayashi H. The constituents of Schizandra chinensis B. IV. isolation and structure determination of five new lignans, gomisin A, B, C F and G, and the absolute structure of schizandrin, Chem. Pharm. Bull. 27(11): 2695, 1979 https://doi.org/10.1248/cpb.27.2695
  14. Ikeya Y. Taguchi H, Yasioka I, and Kobayashi H. The constituents of Schizandra chinensis B. V. isolation and structure determination of five new lignans, gomisin A, B, C F and G, and the absolute structure of schizandrin, Chem. Pharm. Bull. 27(6): 1395, 1979
  15. Folin O, Denis W. On phosphotungastic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-249, 1912
  16. Kang YH, Park YK, Lee GD. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 28: 232-239, 1996
  17. Kim SM, Cho YS, Sung SK. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J. Food Sci. Technol. 33: 626-632, 1996
  18. Song HN, Oh SW. Optimization of extraction and clarification condition for preparation of liquid extract tea from artificially cultivated phellinus linteus. J. Korean Sci. Nutr. 31(4) : 636-641, 2002 https://doi.org/10.3746/jkfn.2002.31.4.636
  19. Lee KE, Oh NS, Park WJ, and Ryu GH. Changes in extraction pattern and yield of bamboo leaf powder at different extraction conditions. Food Industry and Nutrition. 9(1): 46-52, 2004
  20. Lee SE, Bang JK, Seong NS. Inhibitory activity on angiotensin converting enzyme and antioxidant activity of Hovenia dulcis Thunb. Cortex extract. Korean J. Medicinal Crop Sci. 12(1): 79-84, 2004
  21. Jung MS, Lee GS, Chae HJ. Invitro biological activity assay of ethanol extract of Radish. J. Korean Soc. Appl. Biol. Chem. 47(1): 67-71, 2004
  22. Bolis MS, Antioxidant determination by the use of stable free radical. Nature. 26: 1199-1200, 1958
  23. An BJ, Lee JT, Lee SA, Kwak JH, Park JM, Lee JY, and Son JH. Antioxidant effects and application as natural ingredients of korean sanguisorbae officinalis L. J. Korean Soc. Appl. Biol. Chem. 47(2): 244-250, 2004
  24. Lee MJ and Moon GS. Antioxidative effects of korean Bamboo tree, Wang-dae, Som-dae, Maengjong-juk and O-juk. Korean J. Food Sci. Technol. 35(6): 1226-1232, 2003
  25. Marklund S, Marklund G. Involvement of superoxide anion radical in the oxidation of pyrogallol and a cinvenient assay for superoxide dismutase. Eur. J. Biochem. 47: 468-474, 1974
  26. Hong, HD, Kang, NK and Kim SS. Superoxid dismutase-like activity of apple juice mixed with some fruits and vegetables. Korean J. Food Sci Technol. 30: 1484-1487, 1998
  27. Song JH, Lee HS, Hwang JK, Chung TY, Hong SR, and Park KM. Physiological activities of Phellinus ribis extracts. Korean J. Food Sci. Technol. 35: 690-695, 2003 https://doi.org/10.1021/es0011360