초피 용매 추출물의 항산화성 및 항균성에 관한 연구

A Study on the Antioxidative and Antimicrobial Activities of the Chopi(Zanthoxylum Pipperitum DC.) Solvent Extracts

  • 안명수 (성신여자대학교 식품영양학과) ;
  • 김현정 (성신여자대학교 식품영양학과) ;
  • 한미남 (성신여자대학교 식품영양학과) ;
  • 원종숙 (성신여자대학교 식품영양학과)
  • Ahn, Myung-Soo (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Hyun-Jeung (Department of Food and Nutrition, Sungshin Women's University) ;
  • Han, Mi-Nam (Department of Food and Nutrition, Sungshin Women's University) ;
  • Won, Jong-Sook (Department of Food and Nutrition, Sungshin Women's University)
  • 발행 : 2004.04.30

초록

천연첨가물검색의 일환으로 초피의 용매 추출물의 항산화성과 항균성을 실험하고, 소비자의 수용도를 검토하기 위하여 초피를 첨가한 육원전의 관능 검사를 실시한 결과는 다음과 같았다. 1. 초피 용매 추출물들의 옥수수유에 대한 항산화 효과는 가장 많이 사용되는 합성항산화 제인 BHT과 같은 농도에서 BUex는 월등히 높았으며, MEex, EAex는 유사한 경향을 나타냈다. 2. 초피의 용매 추출물의 중 CHex와 BUex가 Escheria coli, Salmonella typhimurium, Psudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus 및 Listeria monocytogenes 모두에 대하여 항균효과를 보였다. 3. 초피 가루를 식품에 첨가하여 제조한 육완자전에 대한 소비자의 기호도를 검사한 결과는 0.1%를 첨가한 경우는 초피를 첨가하지 않은 본래의 육완자전과 같은 수준의 기호도를 나타내므로써(P<0.05) 초피 가루에 의한 이미(異味)가 없어 육와자전 제조에 초피가루를 사용 할 수 있을 것으로 사료된다.

The antioxidative and antimicrobial activity were carried on the Chopi(Zanthoxylum pipperitum) extracts by six kinds of solvents in order to find out new natural food additives. Six solvents were used methanol(MeOH), n-hexan(hexane), chloroform$(CHCl_3)$, ethylacetate(EtOAc), buthanol(BuOH), and water(water) and methanol extract(MEex), hexan extract(HEex), chloroform extract(CHex), ethylacetate extract(EAex), and buthanol extract(BUex), water extract(WAex). The antioxdative activities of them extracts were determined by peroxide value(POV), conjugated diene value(CDV) of corn germ oil storaged for 30 days at $60{\pm}2^{\circ}C$. These extracts were added as 0.02, 0.1, 0.2% of each extracts and compared with ${\alpha}-tocopherol$, and BHT. The antioxidative activities of 0.02% extract were as follows in decreasing order BUex > WAex, BHT, MEex > HEex, EAex, CHex > TOC, and control. While, BUex and CHex among these extracts were shown to be had antimicrobial effects on the microorganism such as Escheria coli, Salmonella typhimurium, Psedomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes. Finally to find out the preference of chopi we had made Yugwanzageon (a kind of shallow fried meat ball)by adding chopi powder. The result were similar to control(not added chopi powder) in case of 0.1% chopi adding Yugwanzageon.(p<0.05) Therefore it was thought to be possible that chopi powder was used Yugwanzageon preperation.

키워드

참고문헌

  1. Beuchut, L.R. and Golden, D.A. : Antimicrobial occurring naturally in food. Food Technol, 43:134, (1989)
  2. Kye Taek Lim, Jae Han Shim Antioxidative Effects of Ethanol Extracts from Rhus Vemidflua Stokes (RVS) on Mouse Whole Brain Cells Korean J. Food Sci. Technol, 29(6): 1248-1254, (1997)
  3. Jang, E. H., Pyo, Y. H. and Ahn, M.S.: Andoxdant effect of omija(Schizandra chinesis Bai11on)extracts. Kor J. Soc. Food Sci., 12(3): 372-376, (1996)
  4. In Chang Jung, Shin Park, Kyung Sook Park, Hyo Cheol Ha, Seon Hee Kim, Yong II Kwon and Jae Sung Lee Andoxidadve Effect of Fruit Body and Mycdial Extracts of Pleurotus ostreatus. Korean J. Food Sci. Technol, 28(3): 464-469, (1996)
  5. Cho S. Y., You B. J., Chang M. H., Lee S. J., Sung N. J. and Lee E. H. :Screening for Antimicrobial Compounds in Unused Marine Resources by the Paper Disk Method. Korean J. Food Sci. Technol.,26(3): 26-165, (1994)
  6. Lee S. W.: Korean food in history, Gyomoonsa, Seoul, p60, (1984)
  7. Yoon S. S. : The history and cooking in Korean foods, Soohagsa. Seoul, p59, (1986)
  8. Lee,S.I.:Bonchohak,Sooseowon,p255,(1981)
  9. Chung, S.K., Jung, J.D. and Cho, S.H. Antimicrobial activity of Chopi(Zanthoxylum pippentum DC.) extract. J. Korean Soc. Food Sci. Nutr. 28(2): 371-377, (1999)
  10. Kim, Y.D., Kang, S.K., Choi, O.J., Lee, H.C., Jang, M.J. and Shin, S.C.: Screening of antimicrobial activity of Chopi(Zanthoxylum pipperitum A.P. DC.)extract. J. Korean Soc. Food Sci. Nutr., 29(6): 1116-1122, (2000)
  11. Park, J.H. Cha, W.S., Oh, S.L, Cho, Y. J., Lee, W.Y. Hur, H.O. Yang, J.M. and Cho, S.G. : Volatile flavor components of Wild Chopi(Zanthoxylum pipperitum DC) leaf. Koiean J. Food and Nutr., 13(5): 483-489, (2000)
  12. Kim, Y.D., Kang, S.K. Choi, O.J. Jung, H.S., Jang, M.J. Seo, J.S. and Ko, M.S.: Change in the chemical compositions of cbopi(Zanthoxylum pipperitum DC.) according to varieties and picking date. J. Korean Soc. Food Sci. Nutr., 30(2): 199-203, (2001)
  13. Ko, Y.S. and Han, H.J. :Chemical constituents of Korean chopi(Zanthoxylum pipperitum DC.) and sancho(Zanthoxylum schinifolium). Korean J. Food Sci. Technol, 28(1): 19-27, (1996)
  14. A.O.A.C. : Official methods of analysis, 15th ed., Assosiadon of official analytical chemists Sodety, Washington,D.C., 994, (1990)
  15. Kim, W. J. : Extraction and Identification of functional compounds, Korean J. Food Sci. Technol, 34(4): 22-28, (2001)
  16. Charles R. Caldwell., Oxygen Radical Absorbance Capacity of the Phenolic Compounds in Plant Extracts Fractionated by High Performance Liquid Chromatography, Analytical Biochemistry, 293: 232-238, (2001) https://doi.org/10.1006/abio.2001.5134
  17. A.O.C.S.: Official and tentative methods, 3rd ed. American oil chemists Society Illinoids, (1978)
  18. Ahn, M.S. : Effects of reaction temperature, time and persence of orgamic acids or their salts on the anfioxdants activity of caramelization mixture, Ph. D. thesis, Korea University, (1984)
  19. davisdon, P.M and Parish, M.E. : Methods for testing the efficacy of food antimicrobials. Food Technol, 1:148, (1989)
  20. Judie, D.D. : Antinucrobial agents. Food Technol. 40:104-110, (1986)
  21. Kim G.O. and Lee Y.C.: The evaluation ot food Hagyeonsa, p353, (1995)