Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제30권3호
  • /
  • Pages.193-202
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  • 2004
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

집토끼 하악골에서 신연 골형성술시 BMP-2,-4의 발현

EXPRESSION OF BMP-2, -4 DURING DISTRACTION OSTEOGENESIS IN THE RABBIT MANDIBLE

  • 김상우 (가톨릭대학교 임상치과학대학원 구강악악면외과학과) ;
  • 지유진 (성빈센트병원 치과 구강악안면외과) ;
  • 송현철 (성빈센트병원 치과 구강악안면외과)
  • Kim, Sang-Woo (Department of Oral & Maxillofacial Surgery, Graduate School of Clinical Dental Science, The Catholic University of Korea) ;
  • Jee, Yu-Jin (Division of Oral & Maxillofacial Surgery, Department of Dentistry, St. Vincent's Hosptital, The Catholic University of Korea) ;
  • Song, Hyun-Chul (Division of Oral & Maxillofacial Surgery, Department of Dentistry, St. Vincent's Hosptital, The Catholic University of Korea)
  • 발행 : 2004.06.30
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초록

Distraction osteogenesis(DO) is defined as a gradual mechanical process of mechanical stretching two vascularized bone surface apart with a critical rate and rhythm such that new bone forms within the expanding gap, reliably bridges the gap, and ultimately remodels to normal structure. DO has become a mainstay in bone tissue engineering and has significantly improved our armamentarium for reconstructive craniomaxillofacial procedures. But the molecular and biological mechanisms that regulate the formation of new bone during distraction osteogenesis are not completely understood. BMPs are potent osteoinductive agents. Our hypothesis was that BMPs, especially BMP-2 and BMP-4, might play an importent role in the signaling pathways that link the mechanical forces created by distraction to biological responses and in promting new bone formation. Using a rabbit's mandible, we investigated the expression of BMP-2, -4 at different time points during distraction osteogenesis. The purpose of this study is to research the pattern of expression of BMP-2, -4 in new bone formation during distraction osteogenesis of the rabbit mandible. The experimental group was applied gradual distraction (0.7mm a day by twice a day, 4.9mm in total, for 7 days) and the control group was carried out osteotomy alone. They were examined clinically, histologically, and by RT-PCR analysis. On 3 days after osteotomy, the high level of expression of BMP-2, -4 was detected. But, the expression of BMP-4 was decreased during latency period. As distraction was started, its expression was increased and maintained till postoperative 28days. In control group, the expression of BMP-4was remarkably decreased till postoperative 14 days. On the other hand, the expression of BMP-2 was no difference between experimental group and control group. The expression of BMP-4 was maintanined at high level during the entire experimental period in both group. These findings suggested that excellent bone formation during distraction osteogenesis is associated with enhanced expression of BMP-4 genes by mechanical tension stress.

키워드

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