Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Screening of antioxidant and antimicrobial activities of Caesalpinia bonducella Flem., leaves (Caesalpiniaceae)

  • Gupta, Malaya (Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Mazumdar, UK (Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Kumar, Ramanathan Sambath (Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Gomathi, Periyasamy (Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Rajeshwar, Y. (Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University) ;
  • Kumar, T. Siva (Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University)
  • Published : 2004.09.30
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Abstract

The study was aimed at evaluating the antioxidant and antimicrobial activities of methanol extract of Caesalpinia bonducella leaves (MECB) (Family: Caesalpiniaceae). The effect of MECB on antioxidant activity, reducing power, free radical scavenging (DPPH radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and hydrogen peroxide radical scavenging), total phenolic content and antimicrobial activities were studied. The antioxidant activity of MECB increased in a dose dependent manner. About 50, 100, 250 and 500 g of MECB showed 53.4, 61.2, 69.1 and 76.2 % inhibition respectively on peroxidation of linoleic acid emulsion. Like antioxidant activity, the effect of MECB on reducing power increased in a dose dependent manner. The free radical scavenging activity of MECB was determined by DPPH radical scavenging method. The potency of this activity was increased with increased amount of extract. MECB was found to inhibit the nitric oxide radicals generated from sodium nitroprusside $(IC_{50}\;=\;102.8\;g/ml)$ whereas the $IC_{50}$ value of curcumin was 20.4 g/ml. Moreover, the MECB was found to scavenge the superoxide generated by photoreduction of Riboflavin. MECB was also found to inhibit the hydroxyl radical generated by Fenton reaction, where the $IC_{50}$ value is 104.17 g/ml compared with catechin 5 g/ml, which indicates the antioxidant activity of MECB. The MECB capable of scavenging hydrogen peroxide in a concentration-dependent manner. The amounts of total phenolic compounds were also determined. Antimicrobial activities of MECB were carried out using disc diffusion methods with five Gram positive, four Gram negative and four fungal species. The results obtained in the present study indicate that MECB leaves are potential source of natural antioxidant and antimicrobial agents.

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References

  1. Alho H, Leinonen J. (1999) Total antioxidant activity measured by chemiluminescence method. Meth. Enzymol. 299, 3-15. https://doi.org/10.1016/S0076-6879(99)99004-3
  2. Aruoma OI. (1998) Nutrition and health aspects of free radicals and antioxidants. Food Chem. Toxicol. 62, 671-683.
  3. Bartner A, Pfeiffer KP, Batner H. (1994) Applicability of disc diffusion methods required by the pharmacopoeias for testing antibacterial activity of natural compounds. Pharmazie 49, 512-516.
  4. Blois MS. (1958) Antioxidant determinations by the use of a stable free radical. Nature 26, 1199-1200.
  5. Davies KJA. (1994) Oxidative stress: the paradox of aerobic life. Biochemistry Society Symphosium 61, 1-34.
  6. DeFeudis FV, Papadopoulos V, Drieu K. (2003) Ginko biloba extracts M Gupta et al. 184 and cancer: a research area in its infancy. Fundam. Clin. Pharmacol. 17, 405-417. https://doi.org/10.1046/j.1472-8206.2003.00156.x
  7. Diplock AT. (1997) Will the 'good fairies' please prove to us that vitamin E lessens human degenerative disease? Free Rad. Res. 27, 511-532.
  8. Duh PD. (1998) Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free radical and active oxygen. J. Am. Oil Chem. Soc. 75, 455-465. https://doi.org/10.1007/s11746-998-0248-8
  9. Duh PD, Tu YY, Yen GC. (1999) Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebnesmittel-Wissenschaft und Technologie 32, 269-277. https://doi.org/10.1006/fstl.1999.0548
  10. El-Habit OHM, Saada HN, Azab KS. (2000) The modifying effect of $\beta$-carotene on gamma radiation-induced elevation of oxidative reactions and genotoxicity in male rats. Mutat. Res. 466, 179-186. https://doi.org/10.1016/S1383-5718(00)00010-3
  11. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JK, Tannenbaum SR. (1982) Analysis of nitrate, nitrite and 15N nitrate in biological fluids. Anal. Biochem. 126, 131-138. https://doi.org/10.1016/0003-2697(82)90118-X
  12. Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OI. (2002) On the in-vitro antioxidant properties of melatonin. J. Pineal. Res. 33, 167-171. https://doi.org/10.1034/j.1600-079X.2002.20920.x
  13. Gulcin I, Oktay M, Kufrevioglu OI, Aslan, A. (2002) Determination of antioxidant activity of Lichen Cetraria islandica (L). Ach. J. Ethnopharmacol. 79, 325-329. https://doi.org/10.1016/S0378-8741(01)00396-8
  14. Gupta M, Mazumder UK, Sambath kumar R, Siva Kumar T, Vamsi MLM. (2004) Antitumor activity and antioxidant status of Caesalpinia bonducella against Ehrlich ascites carcinoma in Swiss albino mice. J. Pharmacol. Sci. 94, 177-184. https://doi.org/10.1254/jphs.94.177
  15. Gupta M, Mazumder UK, Sambath kumar R, Siva kumar T. (2003) Studies on antiinflammatory, analgesic and antipyretic properties of methanol extract of Caesalpinia bonducella leaves in experimental animal models. Iranian J. Pharmacol. Ther. 2, 30-34.
  16. Gupta M, Mazumder UK, Sambath kumar R. (2003) Hepatoprotective and antioxidant role of Caesalpinia bonducella on paracetamol-induced liver damage in rats. Nat. Prod. Sci. 9, 186-191.
  17. Halliwell B. (1991) Reactive oxygen species in living systems: source, biochemistry, and role in human disease. Am. J. Med. 91, 14-22.
  18. Halliwell B, Gutteridge JM. (1989) Free radicals in biology and medicine. Clarendon Press.
  19. Halliwell B, Gutteridge JM. (1999) Free radicals in biology and medicine. Oxford University Press, Oxford.
  20. Halliwell B, Gutteridge JMC, Cross CE. (1992) Free radicals, antioxidants, and human disease: where are we now. J. Lab. Clin. Med. 119, 598-620.
  21. Hatano T, Edamatsu R, Mori A, Fujita Y, Yasuhara. (1989) Effects of interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chem. Pharm. Bull. 37, 2016-2021. https://doi.org/10.1248/cpb.37.2016
  22. Hertog MGL, Feskens EJM, Hollman PCH, Katan MB, Kromhout D. (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: the zupthen elderly study. Lancet 342, 1007-1011. https://doi.org/10.1016/0140-6736(93)92876-U
  23. Kirtikar KR, Basu BD. (1975) Indian Medicinal Plants, Vol. 2, 2nd edn, pp.842-844, Bishen Mahendra Pal Singh, Dehra Dum, India.
  24. Lai LS, Chou ST, Chao WW. (2001) Studies on the antioxidative activities of Hsian-tsao (Mesona procumbens Hemsl) leaf gum. J. Agri. Food Chem. 49, 963-968. https://doi.org/10.1021/jf001146k
  25. Lai MS, Shameel S, Ahmad VU, Ushmanghani K. (1977) Chemical constituents of Caesalpinia bonduc. Pak. J. Sci. Ind. Res. 40, 20-22.
  26. Lyder DL, Peter SR, Tinto WF, Bissada SM, McLean S, Reynolds WF. (1998) Cassane diterpenoids of Caesalpinia bonduc. J. Nat. Prod. 61, 1462-1465. https://doi.org/10.1021/np980198p
  27. Mackeen MM, Ali AM, El-Sharkawy SH, Manap MY, Salleh KM, Lajis NH, Kawazu K. (1997) Antimicrobial and cytotoxic properties of same Malaysian traditional vegetables. Int. J. Pharmacol. 35, 237-243. https://doi.org/10.1076/phbi.35.4.237.13313
  28. Marcocci L Maguire JJ, Droy-Lefaix MT, Packer L. (1994) The nitric oxide scavenging property of Ginkgo biloba extract EGb 761. Biochem. Bioph. Res. Co. 201, 748-755. https://doi.org/10.1006/bbrc.1994.1764
  29. Meir S, Kanner J, Akiri B, Hadas SP. (1995) Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agri. Food Chem. 43, 1813-1815. https://doi.org/10.1021/jf00055a012
  30. Mistuda H, Yuasumoto K, Iwami K. (1996) Antioxidation action of indole compounds during the autooxidation of linoleic acid. Eiyo to Shokuryo 19, 210-214.
  31. Nishimiki M, Rao NA,Appaji N, Vagi K. (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem. Bioph. Res. Co. 46, 849-853. https://doi.org/10.1016/S0006-291X(72)80218-3
  32. Ohkawa H, Ohishi N, Yagi K. (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  33. Okuda T, Kimura Y, Yoshida T, Hatano T, Okuda H, Arichi S. (1983) Studies on the activities of tannins and related compounds from medicinal plants and drugs. I. Inhibitory effects on lipid peroxidation on mitochondria and microsomes of liver. Chem. Pharm. Bull. 31, 1625-1631. https://doi.org/10.1248/cpb.31.1625
  34. Oyaizu M. (1986) Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutrition 44, 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  35. Peter SR, Tinto WF, Lean S, Reynolds WF, Yu M (1997) Bonducellpins A-D new cassane furanoditerpens of Caesalpinia bonduc. J. Nat. Prod. 60, 1219-1221. https://doi.org/10.1021/np970308c
  36. Purushothaman KK, Kalyani K, Subramaniam K, Shanmughanathan SP. (1982) Structure of bonducellin-A new homoisoflavone from Caesalpinia bonducella. Indian J. Chem. 21B, 383-386.
  37. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. (1995) The relative antioxidant activity of plant derived polyphenolic flavanoids. Free Rad. Res. 22, 375-383. https://doi.org/10.3109/10715769509145649
  38. Robinson EE, Maxwell SRJ, Thorpe GHG. (1997) An investigation of antioxidant activity of black tea using enhanced chemiluminescence. Free Rad. Res. 26, 291-302. https://doi.org/10.3109/10715769709097807
  39. Ruch RJ, Cheng SJ, Klaunig JE. (1989) Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10, 1003-1008. https://doi.org/10.1093/carcin/10.6.1003
  40. Slinkard K, Singleton VL. (1977) Total phenol analyses; Automation and comparision with manual methods. Am .J. Enol. Vitic. 28, 49-55.
  41. Soares, JR, Dins TCP, Cunha AP, Ameida LM. (1997) Antioxidant activity of some extract of Thymus zygis. Free Rad. Res. 26, 469-478. https://doi.org/10.3109/10715769709084484
  42. Tanaka M, Kuei CW, Nagashima Y, Taguchi T. (1998) Application of antioxidative maillrad reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi. 54, 1409-1414.
  43. Tanizawa H, Ohkawa Y, Takino Y, Ueno A, Kageyama T, Hara S. (1992) Studies on natural antioxidants in citrus species I. Determination of antioxidant activities of citrus fruits. Chem. Pharm. Bull. 40, 1940-1942. https://doi.org/10.1248/cpb.40.1940
  44. Takeoka GR, Dao LT. (2003) idant constituent of almond [Prunus dulcis (Mill.) D.A. Webb.] hulls. J. Agric. Food Chem. 51, 496-501. https://doi.org/10.1021/jf020660i
  45. Ye XY, Wang HX, Liu F, Ng TB. (2000) Ribonuclease, cell-free translation-inhibitory and superoxide radical scavenging activities of the iron-binding protein lactoferrin from bovine mill. Int. J. Biochem. Cell Biol. 32, 235-241. https://doi.org/10.1016/S1357-2725(99)00131-4
  46. Yen GH, Chen HY. (1995) Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agri. Food Chem. 43, 27-32. https://doi.org/10.1021/jf00049a007
  47. Yen GC, Duh PD. (1994) Scavenging effect of methanolic extracts of peanut hulls on free radical and active oxygen. J. Agri. Food Chem. 42, 6-29.
  48. Yildirim A, Oktay M, Bilaloglu V. (2001) The antioxidant activity of the leaves of Cydonia vulgaris. Turkish J. Med. Sci. 31, 23-27.
  49. Yildirim A, Mavi A, Kara AA. (2001) Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J. Agri. Food Chem. 49, 4083-4089. https://doi.org/10.1021/jf0103572
  50. Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V. (2000) Comparison of antioxidant and antimicrobial activities of tilia (Tilia argenta Desf Ex DC), sage (Salvia triloba L.) and black tea (Camellia sinensis) extracts. J. Agri. Food Chem. 49, 5030-5034.