Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibitory Activity of the Extracts from the Pigmented Rice Brans on Inflammatory Reactions

유색미 겨 추출물의 염증반응 억제활성

  • Choi, Sun-Phil (Department of Biological Science, Ajou University) ;
  • Kang, Mi-Young (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Nam, Seok-Hyun (Department of Biological Science, Ajou University)
  • Published : 2004.06.30
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Abstract

Effects of the extracts from bran part of the pigmented rices on inflammation was evaluated by determining their inhibitory action on the production of nitric oxides, histamines and matrix metalloproteinase(MMP) from inflammatory leukocytes. Effects on the production of nitric oxides in a macrophage cell line, RAW264.7 cells, were determined, demonstrating that any significant difference was not detected between the normal rice and the pigmented rice extracts. Inhibitory effects on the histamine-release from a basophilic cell line, RBL-2H3, were examined, showing 3.6 to 5.4-fold increase in the inhibitory activity compared to that of the normal rices. Among the pigmented rice cultivars tested, especially, inhibitory activity of LK1-3-6-12-1-1 was the greatest. Using RAW264.7 cells, we examined the effect of the pigmented rice extracts on the MMP activity. The results showed that the enzyme activity increased with the increasing concentration of the normal rice extract. However, the pigmented rice extracts, except LK1A-2-12-1-l, acted to decrease the MMP activity with their increasing concentrations. The results described above showed the superiority of the pigmented rice extracts in inhibition on release of histamine and MMP, pivotal factors for causing inflammatory responses, from the leukocytes.

유색미 겨 추출물이 염증반응에 미치는 효과를 백혈구가 분비하는 염증관련 산물인 nitric oxide, histamine 및 matrix metalloproteinase(MMP) 생성에 대한 억제활성을 측정하여 평가하였다. 대식세포 세포주인 RAW264.7 세포에서 nitric oxide에 미치는 효과를 측정한 결과, 일반미와 유색미 추출물 간에 큰 활성의 차이를 보이지 않았다. 호중구 세포주인 RBL-2H3 세포를 이용하여 histamine 분비에 대한 억제활성을 조사한 결과, 유색미 추출물이 일반미에 비해서 약 3.6-5.4배 정도의 우수한 저해능을 보였다. 특히 조사한 유색미 5품종 중 LK1-3-6-12-1-1가 저해활성이 가장 높았다. RAW264.7 세포주를 이용하여 유색미 추출물이 matrix metalloproteinase(MMP) 활성에 미치는 영향을 조사하였다. 그 결과, 일반미는 농도의 증가에 따라 오히려 효소활성이 증가한 반면, 유색미 경우는 LK1A-2-12-1-1을 제외하고 농도의 증가에 따라 효소활성이 감소하는 것을 관찰할 수 있었으며, 특히 LK1-3-6-12-1-1와 Elwee의 저해활성이 가장 높았다. 이상의 결과는 유색미가 염증반응의 원인인 백혈구의 histamine과 MMP의 분비를 저해하는 능력이 일반미보다 우수함을 나타내었다.

Keywords

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