Applied Biological Chemistry

  • 제47권2호
  • /
  • Pages.170-175
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  • 2004
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

Bacillus sp. TBM40-3에 의해 생성된 Biosurfactant의 유류분해 특성

Characterization of Oil-Degradation Biosurfactant Produced by Bacillus sp. TBM40-3

  • 김선희 (동아대학교 응용생명공학부) ;
  • 이상철 (동아대학교 응용생명공학부) ;
  • 유주순 (동아대학교 응용생명공학부) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 정수열 (동주대학 식품과학) ;
  • 최용락 (동아대학교 응용생명공학부)
  • Kim, Sun-Hee (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Sang-Cheol (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Yoo, Ju-Soon (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Joo, Woo-Hong (Dept. of Biology, Chang-Won University) ;
  • Chung, Soo-Yeol (Dept. of Food Science, Dong-Ju College) ;
  • Choi, Yong-Lark (Department of Biotechnology Faculty of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2004.06.30
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초록

환경친화적이며 원유 분해능이 강력한 균주를 얻기 위하여, 태백산의 토양 일부를 채취하였다. 채취한 토양 sample 로부터 crude oil을 탄소원으로 이용하는 수십 종의 균주를 분리하였으며, 분리된 균주 중 원유분해능 및 biosurfactant 생성능이 가장 우수한 균주를 선별하였다. 또한 분리된 균주의 형태학적 특성을 관찰하고 165 rDNA sequence를 통하여 Bacillus sp. TBM40-3로 동정하였다. 또한 phylogenetic tree를 이용하여 이미 동정된 다른 균주들과의 유연관계를 파악하였다. Bacillus sp. TBM40-3의 배양액의 표면장력은 최저 29 mN/m까지 감소되었고, 생산된 biosurfactant의 유화활성은 soybean oil에서 최대였으며, crude oil에서도 높은 편이였다. 유화안정성은 합성계면활성제와 비슷하거나 우수하였다. 따라서 TBM40-3에서 추출한 biosurfactant는 합성계면활성제를 대체할 수 있는 환경친화적인 생물 계면활성제로 사용될 수 있는 가능성을 보여준다.

In this paper we studied about Bacillus sp. TBM40-3 producing biosurfactants. The strains were isolated from Taeback Mountain soil and identified as Bacillus sp. by l6S rDNA nucleotides sequence analysis. The TBM40-3 was gram-positive and rod-shaped as observed by field emission scanning microscopy. After the cultivation TBM40-3 in LB broth for 90 h and the surface tension of supernatant was decreased to 29 mN/m. Emulsification activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oil as substrate. Maximum emulsification activity and stability was obtained from soybean oil. Also, we confirmed that the TBM40-3 producing biosurfactant had an effect on crude oil while showing a superior effect as compared to chemically synthesized surfactants (SDS, Span85, Tween40, Triton X-100). As a result, the Bacillus sp. TBM40-3 producing biosurfactant had potent properties as an emulsifying agent and an emulsion stabilizing agent.

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