Journal of Pharmaceutical Investigation

  • 제34권4호
  • /
  • Pages.305-310
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  • 2004
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  • 2093-5552(pISSN)
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  • 2093-6214(eISSN)
한국약제학회 (The Korean Society of Pharmaceutical Sciences and Technology)
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음이온성 리포솜의 방출 거동과 안정성

Release Profile and Stability of Anionic Liposomes

  • 남다은 (충북대학교 화학공학과) ;
  • 한희동 (한국화학연구원 나노생체재료연구원) ;
  • 박윤정 (한국화학연구원 나노생체재료연구원) ;
  • 김윤아 (한국화학연구원 나노생체재료연구원) ;
  • 신병철 (한국화학연구원 나노생체재료연구원)
  • Nam, Da-Eun (Department of Chemical Engineering, Chungbuk National University) ;
  • Han, Hee-Dong (Nanobiomaterials Lab. Korea Research Institute of Chemical Technology) ;
  • Park, Yun-Jung (Nanobiomaterials Lab. Korea Research Institute of Chemical Technology) ;
  • Kim, Yun-A (Nanobiomaterials Lab. Korea Research Institute of Chemical Technology) ;
  • Shin, Byung-Cheol (Nanobiomaterials Lab. Korea Research Institute of Chemical Technology)
  • 발행 : 2004.08.20
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초록

This study was to prepare the anionic liposomes which were to release anticancer drug (doxorubicin) at the hyperthermia temperature $({\sim}42^{\circ}C)$ and to stabilize in bovine serum solution at $37^{\circ}C$. The vesicle size and zeta potential of liposomes in Tris-HCl buffered solution (pH 7.4) were measured by an electrophoretic light scattering spectrophotometer. To estimate the stability of liposomes, liposome size was measured in bovine serum solution at $37^{\circ}C$ for 72 h. The release of doxorubicin from liposome was determined by measuring the fluorescence intensity using fluorescence spectrophotometry with temperature and time. The size of liposomes was from 120 to 160 nm and zeta potential was from $-33.3{\pm}2.4$ to $-75.6{\pm}6.9\;mV$. Anionic liposome was stabilized in bovine serum solution at $37^{\circ}C$ within 72 h. Additionally, the release transition temperature of doxorubicin from liposomes was increased by increasing mole % of anionic phospholipid.

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