한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제12권5호
  • /
  • Pages.378-383
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  • 2004
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

Effects of Elicitors on Scopolamine Production of Scopolia parviflora Nakai Adventitious Roots in Bubble Column Bioreactor

  • Jung, Hee-Young (Division of Forest Science, Gyeongsang National University) ;
  • Kim, Won-Jung (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Seung-Mi (Division of Forest Science, Gyeongsang National University) ;
  • Park, Dong-Jin (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Division of Forest Science, Gyeongsang National University) ;
  • Choi, Myung-Suk (Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.)
  • 발행 : 2004.11.01
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초록

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

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참고문헌

  1. Aoki J, Matsumoto H, Asako Y, Matsunaga Y, Shimomura K (1997) Variation of alkaloid productivity among several clones of hairy roots regenerated plants of Atropa beIladonna with Agrobacterium rhizogenes 15834. Plant Cell Rep. 16:282-286
  2. Endo T, Yamada Y (1985) Alkaloid production in cultured roots of three species of Duboisia. Phytochemistry 24:1233-1236 https://doi.org/10.1016/S0031-9422(00)81107-3
  3. Jung HY, Kang MJ, Kang YM, Yun DJ, Bahk JD, Chung YG, Choi MS (2002) Optimal culture conditions and XAD resin on tropane alkaloid production in Scopolia parviflora hairy root cultures. Kor. J. Biotechnol. Bioeng. 17:525-530.
  4. Jung HY, Kang SM, Kang YM, Kim YD, Yang JK, Chung YG, Choi MS (2003a) Selection of optimal biotic elicitor on tropane alkaloid production of hairy roots in Scopolia parviflora Nakai. Kor. J. Medicinal. Crop Sci. 11: 358-363
  5. Jung HY, Kang SM, Kang YM, Kang MJ, Yun DJ, Bahk JD, Yang JK, Choi MS (2003b) Enhanced production of scopolamine by bacterial elicitors in adventitious hairy root cultures of Scopolia paIYif1ora. Enzyme Microb. Technol. 33: 987-990 https://doi.org/10.1016/S0141-0229(03)00253-9
  6. Kamada H, O kamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep. 5:239-242 https://doi.org/10.1007/BF00269811
  7. Kang SM, lung HY, Kang YM, Yun DJ, Bahk JD, Yang JK, Choi MS (2004) Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia paIYiflora. Plant Sci. 166:745-751 https://doi.org/10.1016/j.plantsci.2003.11.022
  8. Pitta-alvarez SI, Spollansky TC, Giulietti AM (2000) Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline. Biotechnol. Lett. 22:16531656 https://doi.org/10.1023/A:1005638029034
  9. Richards FJ, Coleman EG (1952) Occurrence of putrescine in potassium deficient barley. Nature 170:460-461
  10. Yamada Y, Tabata M (1997) Plant biotechnology of tropane alkaloids. Plant Biotech. 14:1-10
  11. Yukimune Y, Hara Y, Yamada Y (1994) Tropane alkaloid production in root cultures of Duboisia myoporoides obtained by repeated selection. Biosci. Biotech. Biochem. 58:1443-1446 https://doi.org/10.1271/bbb.58.1443
  12. Zabetakis I, Edwards R, Hamilton JTG, O'Hagan D (1998) The biosynthetic relationship between littorine and hyoscyamine in transformed roots of Datura stramonium. Plant Cell Rep. 18:341-345 https://doi.org/10.1007/s002990050583