Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of Elicitors on Scopolamine Production of Scopolia parviflora Nakai Adventitious Roots in Bubble Column Bioreactor

  • Jung, Hee-Young (Division of Forest Science, Gyeongsang National University) ;
  • Kim, Won-Jung (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Seung-Mi (Division of Forest Science, Gyeongsang National University) ;
  • Park, Dong-Jin (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Division of Forest Science, Gyeongsang National University) ;
  • Choi, Myung-Suk (Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.)
  • Published : 2004.11.01
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Abstract

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

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References

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