Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Extracts from Pinus densiflora Siebold et Zuccarini Sawdust Inhibit the Mycelial Growth of Lentinus edodes (Berk.) Sing., Edible Mushroom

  • Hong, Seong-Cheol (Faculty of Forest Sci. & Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.) ;
  • Choi, Myung-Suk (Faculty of Forest Sci. & Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.) ;
  • Lim, Bu-Kug (Department of Forest Science & Technology, Kyungpook Natl. Univ.) ;
  • Lee, Jong-Yoon (Department of Forest Science & Technology, Kyungpook Natl. Univ.) ;
  • Rinker, Danny L. (Department of Plant Agriculture, University of Guelph) ;
  • Yang, Jae-Kyung (Faculty of Forest Sci. & Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.)
  • Published : 2004.11.01
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Abstract

The extracts of Pinus densiflora sawdust by n hexane, ethyl acetate and methanol solvent were investigated to identify their mycelial growth inhibition against Lentinus edodes. The yields of n hexane soluble fraction, ethyl acetate-soluble fraction, and methanol soluble fraction from P. densiflora sawdust were obtained 1.36%, 2.21% and 4.03% using organic solvent, respectively. The mycelial growth inhibition of L. edodes was the greatest for n hexane extract, ranging from 36.5% to 47.6% at concentrations of 125 ppm to 1,000 ppm, with the values for all concentrations significantly different from one another. After direct extraction of P. densiflora sawdust using n hexane, ethyl acetate and methanol, each extract was separated into three fractions by silica gel column chromatography and then the fractions were isolated on the values of $R_f$ by thin layer chromatography. The mycelial growth inhibition against L. edodes was recognized in the fractions II (33.5%) and III (37.6%) of n hexane extract, the fraction II (21.4%) of ethyl acetate extract and the fraction II (26.4%) of methanol extract. The fractions III of n-hexane extract showed the highest growth inhibition among the nine fractions of the organic solvent extract.

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References

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