Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Antioxidant Activity of Ulmus davidiana var. japonica N. and Hemipteleae davidii P.

느릅나무와 시무나무의 항산화 활성

  • Published : 2004.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cortex of Ulmus davidiana var. japonica N. and Hemipteleae davidii P. have been used without classification according to countries as oriental medicine for treatment of various disorders. This study was conducted to elucidate the difference in antioxidant activity and total phenol content of these medicinal materials. Root cortex and trunk cortex of two medicinal plants were extracted with 80% ethanol $(at\;85^{\circ}C)$ and water (at room temperature), respectively. All of the extracts at $200{\sim}5\;{\mu}g/ml$, showed more effective scavenging activity on superoxide radical than ascorbic acid, and 80% ethanol and water extracts of Ulmus root cortex (URC) was more effective. Scavenging activities on DPPH radical of 80% ethanol extracts from URC and Ulmus trunk cortex (UTC) at 10 ${\mu}g/ml$, (41.4, 35.6%) were higher than those of the other six extracts and ${\alpha}-tocopherol\;(2.3{\sim}24.0%)$. Inhibitory activities on human low density lipoprotein (LDL) oxidation of 80% ethanol extracts from UTC and URC (84.3, 81.7%) at $10\;{\mu}g/ml\;(31.3{\sim}78.2%)$ were higher than those of the other six extracts and ${\alpha}-tocopherol$. However, antioxidant activities on linoleic acid peroxidation of all of the extracts were relatively lower than that ${\alpha}-tocopherol$. Total phenol content of 80% ethanol extract from URC was the highest value as 50.8% and that of water extract from HRC was the lowest as 5.6% among the extracts. From these results, it is suggested that Ulmus root cortex was the most effective in antioxidant activity.

한약재로서 혼용되고 있는 느릅나무 및 시무나무의 수피와 근피의 항산화활성 및 총페놀 함량의 차이를 구명하고자 본 연구를 수행하였으며 그 결과는 다음과 같다. DPPH 라디칼에 대해 느릅나무 근피와 수피의 80% 에탄올 추출물은 $10\;{\mu}g/ml$의 농도에서도 각각 41.4% 및 35.6%의 활성을 보여 다른 추출물의 활성 $(2.3{\sim}24.0%)$에 비해 우수한 효과를 가지는 것을 알 수 있었고, superoxide 라디칼에 대해서는 $200{\sim}5\;{\mu}g/ml$의 농도에서 느릅나무 및 시무나무의 수피와 근피로부터 얻어진 모든 추출물이 항산화제인 ascorbic acid보다 우수한 활성을 나타내었으며 특히 느릅나무 근피의 80% 에탄올 추출물과 물 추출물이 좀 더 높은 활성을 나타내었다. 사람의 저밀도지단백 (LDL) 산화에 대한 실험 결과 느릅나무 수피 및 근피의 80% 에탄올 추출물이 $10\;{\mu}g/ml$의 농도에서 84.3% 및 81.7%로 다른 추출물들의 활성 $(31.3%{\sim}78.2%)$보다 비교적 높은 것을 확인할 수 있었다. 실험된 모든 추출물은 linoleic acid 과산화에 대해서 ${\alpha}-tocopherol$보다 낮은 저해활성을 나타내었다. 한편, 총페놀 함량은 느릅나무 근피의 80% 에탄올 추출물이 50.8%로 가장 높았으며 시무나무 근피의 물 추출물이 5.6%로 가장 낮았다. 이상의 실험결과, 느릅나무의 근피와 수피가 시무나무의 근피와 수피보다 더 효과적인 항산화 활성을 보유하며 특히, 느릅나무 근피의 80% 에탄올 추출물은 가장 항산화 활성이 우수한 것으로 확인되었다.

Keywords

References

  1. Bae YS, Kim JK (2000) Extractives of the bark of ash and elm as medicinal hardwood tree species. Mokchae Konghak 28:62-69
  2. Bloi MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  3. Chang BS, Kwon YS, Kim CM (2004) The chemical structures and their antioxidant activity of the components isolated from the heartwood of Hemiptelea davidii. Korean J. Pharmacogn. 35:80-87
  4. Cho SK, Lee SG, Kim CJ (1996) Anti-inflammatory and analgesic activities of water extract of root bark of Ulmus parvifolia. Korean J. Pharmacogn. 27:274-281
  5. Dasgupta N, De B (2004) Antioxidant activity of Piper betle L. leaf extract in vitro. Food Chemistry (In press) https://doi.org/10.1016/j.foodchem.2004.01.036
  6. Eun JS, Song WY (1994) The combined effects of n-BuOH fraction of Ulmi cortex and anticancer drugs on cancer cell lines. Korean J. Pharmacogn 25:144-152
  7. Gaut JP, Heinecke JW (2001) Mechanisms for oxidizing low density lipoprotein. Trends Cardiovac. Med. 11:103-112 https://doi.org/10.1016/S1050-1738(01)00101-3
  8. Gugliucci A, Menini T (2002) Three different pathways for human LDL oxidation are inhibited in vitro by water extracts of the medicinal herb. Life Science 71:693-705 https://doi.org/10.1016/S0024-3205(02)01734-4
  9. Haraguchi H, Hashimoto K, Yagi A (1992) Antioxidative substances in leaves of Polygonum hydropiper. J. Agric. Food Chem. 40:1349-1351 https://doi.org/10.1021/jf00020a011
  10. Hong ND, Rho YS, Kim NJ, Kim JS (1990) A study on efficacy of Ulmi cortex. Korean J. Pharmacogn. 21:217-222
  11. Hong ND, Rho YS, Kim NJ, Kim JS (1990) Studies on the constituents of Ulmi cortex. Korean J. Pharmacogn. 21:201-204
  12. Kim CS, Lee JM, Choi CO, Park SB, Eom TJ (2002) Chemical analysis and isolation of antibacterial compound from Ulmus species ( I ): chemical analysis and antibacterial activity of extractives. Mokchae Konghak 30:66-73
  13. Kim CS, Lee JM, Choi CO, Park SB, Eom TJ (2003) Chemical analysis and isolation of antibacterial compound from Ulmus species (II): isolation and chemical structure of antibacterial compound. Mokchae Konghak 31:16-21
  14. Kim J, Choi M, Cho J, Jung Y, Park T (1994) Effect of Eunymus alatus and Ulmus davidiana var. japonica on the immune system. Korean J. Vet. Res. 34:307-313
  15. Kim NM, Sung HS, Kim WJ (1993) Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci. Technol. 25:204-209
  16. Kim SH, Hwang KT, Park JC (1992) Isolation of flavonoids and determination of rutin from the leaves of Ulmus parviflora. Korean J. Pharmacogn. 23:229-234
  17. Kwon YM, Lee JH, Lee MW (2002) Phenolic compounds from barks of Ulmus macrocarpa and its antioxidative activities. Korean J. Pharmacogn. 33:404-410
  18. Lee EB, Jung KW, Jung CS, Kim OK (1995) The influence of methanol extract of Ulmus davidiana var. japonica cortex on gastric erosion and ulcer and paw edema in rats. Yakhak Hoeji 39:671-675
  19. Lee HY, Kim CK, Sung TK, Mun TK, Lim CJ (1992) Antibacterial activity of Ulmus pumila L. extract. Korean J. Appl. Microbiol. Biotechnol. 20:1-5
  20. Lee KH, Jeon EK, Yoo SY, Oh MJ (2000) Antioxidative activity of Ulmi cortex extract. Korean J. Postharvest Sci. Technol. 7:373-379
  21. Lee S, Lee C, Kim I, Ruy J, Lim J, Kim S, Choi S, Kim S, Oh Y, Kim S (2001) Tumor suppress effects of extracts from Ulmus pumila, Eriobotrya japonica, and Artemisia capillaris Thunb in nude mice. J. Korean Association of Cancer Prevention 6:19-25
  22. Lee YJ, Han JP (2000) Antioxidative activities and nitric scavenging abilities of extracts from Ulmus devidiana. J. Korean Soc. Food Sci. Nutr. 29:893-899
  23. Lim JP, Cui X (2002) Effect of water extract of Ulmi pumilae cortex on gastric ulcer in rats. Korean J. Medicinal Crop Sci. 10:177-180
  24. Miller CP, Jirkovsky I, Hayhurst DA, Adelman SJ (1996) In vitro antioxidant effects of estrogens with a hindered 3-OH function on the copper-induced oxidation of low density lipoprotein. Steroids 61:305-308 https://doi.org/10.1016/0039-128X(95)00234-H
  25. Nishikimi M, Rao NA, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Biophys. Res. Commun., 46:849-854 https://doi.org/10.1016/S0006-291X(72)80218-3
  26. Park JS, Shim CJ, Jung JH, Lee GH, Sung CK, Oh MJ (1999) Antimicrobial activity of Ulmi cortex extracts. J. Korean Soc. Food Sci. Nutr. 28:1022-1028
  27. Vaya J, Mahmood S, Goldblum A, Aviram M, Volkova N, Shaalan A, Musa R, Tamir S (2003) Inhibition of LDL oxidation by flavonoids in relation to their structure and calculated enthalpy. Phytochemistry 62:89-99 https://doi.org/10.1016/S0031-9422(02)00445-4
  28. Yang YL, Kim YJ (2001) Immunostimulating exopolysaccharide with anticancer activity from Enterobacter sp. SSYL (KCTC 0687BP) screened from Ulmus parvifolia. Korean J. Biotechnol. Bioeng. 16:554-561
  29. 江蘇新醫學院 (1979) 中藥大解典. 商務印書館印行. 香港. p. 1264-1265, 2438-2439
  30. 한국의약품시험연구소 (2000) 한약(생약)규격집. 대명기획. 서울. p. 367
  31. 조선인민주의인민공화국 약전위원회 (1996) 조선민주주의인민공화국약전. 의과학출판사. 평양. p. 134
  32. 行政玩 衛生暑 (1985) 중화민국中藥典範. 達昌印刷有限公司. 臺北. p. 278-279
  33. 國家中醫藥管理局 (1999) 中華本草 2卷. 上海科學技術出版社. 上海. p. 445. 453-455
  34. 黃宮潚 (1997) 本草求眞. 의성당. 서울. 12:113-133
  35. 李時珍 (1982) 本草綱目. 人民衛生出版社. 北京. p. 2040-2041