한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제12권3호
  • /
  • Pages.255-261
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  • 2004
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

목단피의 세포독성 물질

A Cytotoxic Compound from Moutan Cortex Radicis

  • Chu, Bo-Yeun (College of Pharmacy, Chungnam National University) ;
  • Jin, Wen-Yi (College of Pharmacy, Chungnam National University) ;
  • Choi, Su-La (College of Pharmacy, Chungnam National University) ;
  • Myung, Pyung-Keun (College of Pharmacy, Chungnam National University) ;
  • Seong, Yeon-Hee (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk Natl. Univ.) ;
  • Song, Kyung-Sik (College of Agriculture & Life Sci., Kyungpook Natl. Univ.) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
  • 발행 : 2004.06.30
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초록

21 종의 생약 추출물을 선정하여 $5\;{\mu}g/ml$의 농도에서 SK-MEL-28 cell 에 대한 세포독성을 조사한 결과 목단피의 메탄올 추출물이 74.3%의 성장율을 보였다. 활성 물질을 찾기 위하여 목단피로부터 silica gel column chromatography를 실시하여 hexane 분획과 EtOAc 분획에서 충 5개의 화합물을 분리하였고 $mp,\;UV,\;IR,\;^1H-NMR,\;^{13}C-NMR$ 등 각종 물리, 화학적 data로부터 그 구조를 paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy-acetophenone (4), paeoniflorin (5)으로 동정하였다. 분리한 물질을 human 피부 암세포인 SK-MEL-28 세포주에 대하여 $10\;{\mu}g/ml$의 농도에서 SRB방법으로 세포독성을 측정한 결과 compound 4가 $ED_50$ 값이 $5.92\;{\mu}g/ml$로 가장 좋은 세포독성을 나타내었다. 이결과는 compound 4가 SK-MEL-28 melanoma 세포주에 대한 새로운 항암 후보 물질임을 제시한다.

The MeOH extracts from 21 species were tested for their cytotoxicity against SK-MEL-28 melanoma cell line and HaCat normal cell line in 5 g/ml by sulforrhodamine-B (SRB) method. Among them, the MeOH extract from Moutan Cortex Radicis showed the moderate activity with the growth rate of 74.3% in SK-MEL-28 cells and the high activity with the growth rate of 207.8% in HaCat cells. Activity-guided fractionation was performed and five compounds, paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy- acetophenone (4), paeonfliorin (5) were isolated from hexane and EtOAc fraction. The structures were established by physicochemical and spectrometric methods $(mp,\;UV,\;IR,\;^1H-NMR,\;^{13}C-NMR,\;spectram)$. All compounds were evaluated for their cytotoxicity, compound 4 showed the significant cytotoxic activity with $ED_50$ value of $5.92\;{\mu}g/ml$, but the other compounds no activity. These results suggest that compound 4 is a novel anticancer candidate against SK-MEL-28 melanoma cells.

키워드

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