한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제12권3호
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  • Pages.237-242
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  • 2004
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

인삼의 부위별 항산화 활성

Antioxidant Activities of Leaf, Stem and Root of Panax ginseng C. A. Meyer

  • 발행 : 2004.06.30
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초록

인삼 식물체의 식품 첨가물로의 이용성을 타진하기 위해 각 부위별 항산화 활성 및 총 페놀 함량을 분석, 비교하였으며 그 결과는 다음과 같다. 인간 LDL의 산화에 대한 저해활성은 뿌리 $(45.2{\sim}54.3%)$에서 높은 활성을 나타내었으며, linoleic acid에 대한 자동산화에 대해서는 잎이 $90.1{\sim}96.5%$의 매우 높은 저해활성을 나타내었다. Superoxide anion 라디칼에 대해서 줄기 $(35.6{\sim}76.1%)$, 잎 $(60.1{\sim}69.3%)$, 뿌리 $(-5.6{\sim}20.1%)$의 순으로 높은 활성을 나타내었다. DPPH 라디칼 소거능은 대체적으로 낮은 활성을 나타내었으며 총 페놀 화합물은 인삼 잎 $(147{\sim}200\;mg%)$, 줄기 $(110{\sim}153\;mg%) $, 뿌리 $(61{\sim}86\;mg%)$의 순으로 높게 나타났다.

To develop a new functional material, leaf, stem and root of Panax gjnseng were analyzed in their antioxidant activities. Root and leaf of ginseng collected from 3 regions, exhibited inhibition activity as $45.2{\sim}54.3%\;and\;90.1{\sim}96.5%$ on peroxidation of low density lipoprotein and linoleic acid, respectively. Scavenging activities of stem, leaf and root of ginseng on superoxide anion radical were $35.6{\sim}76.1%,\;60.1{\sim}69.3%\;and\;-5.6{\sim}20.1%$, respectively. Total phenol contents of leaf, stem and root of ginseng were $147{\sim}200\;mg%,\;110{\sim}153\;mg%\;and\;61{\sim}86\;mg%$ respectively as tannic acid equivalent.

키워드

참고문헌

  1. Bioi MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  2. Cha JY, Kim HJ, Chung CH, Cho YS (1999) Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr, 28:1310-1315
  3. Cho MZ, Hahn TS, Kwon TB, Oh SK (1989) Antioxidant effect of some chelating agents on soybean oil. J. Korean Agric. Chem. Soc, 32:30-36
  4. Chai KJ, Kim MW, Hong SK, Kim DH (1983) Effect of solvents on the yield, brown color intensity, UV absorbance, reducing and antioxidant activities of extracts from white and red ginseng. J. Korean Agric, Chem. Soc. 26:8-18
  5. Choi KJ, Kim SC, Kim MW, Nam KY (1987) Identification of saponin and sapogenin in root, leaf and stem of ginseng by thin layer chromatography. J. Korean Agric. Chem, Soc.30:340-344
  6. Haraguchi H, Hashimoto K, Yagi A (1992) Antioxidative substances in leaves of Polygonum hydropiper. J. Agric. Food Chern. 40:1349-1351 https://doi.org/10.1021/jf00020a011
  7. Jeon HK, Kim SC, Jung NP (1991) Effects of ginseng saponin fraction and cyclophosphamide on the tumoricidal activity of mouse macrophage and the antitumor effect. Korean J.Ginseng Sci. 15:99-105
  8. Joo CN, Kim JH (1992) Study on the hypoglycemic action of ginseng saponin on streptozotocin induced diabetic rats ( I ). Korean J. Ginseng Sci. 16: 190-197
  9. Kang SY, Kim ND (1992) The antihypertensive effect of red ginseng saponin and the endothelium-derived vascular relaxation. Korean J. Ginseng Sci. 16:175-182
  10. Kanner J, Mendel H (1977) Prooxidant and antioxidant effects of ascorbic acid and metal salts in a ${\ss}-carotene-linoleate$,model system, J, Food Sci, 42:60-64 https://doi.org/10.1111/j.1365-2621.1977.tb01218.x
  11. Kim JS, Kim KW, Choi KJ, Kwak YK, 1m KS, Lee KM, Chung BY (1996) Screening of antioxidative components from red ginseng saponin, Korean J. Ginseng Sci, 20:173-178
  12. Kim MJ, Jung NP (1987) The effect of ginseng saponin on the mouse immune system. Korean J. Ginseng Sci. 11:130-135
  13. Kim NM, Sung HS, Kim WJ (1993) Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci, Technol, 25:204-209
  14. Kim KH, Lee YS, Jung IS, Park SY, Chung BY, Lee IR, Yun YS (1998) Acidic polysaccharide from Panax ginseng, ginsan, induces Th1 cell and macrophage cytokines and generates LAK cells in synergy with rII-2. Planta Medica 64:110-115 https://doi.org/10.1055/s-2006-957385
  15. Kim SI, na JY, Jo DH, Lee CY (1987) Extraction and purification of ginseng oligopeptides with antilipolytic actvities, Korean Agric. Chem Soc, 30:88-94
  16. Kitagawa I (1983) Chemical studies on crude drug processing.I . On constituents of ginseng radix rubra (1), Yakugaku Zasshi 103:612-622
  17. Kiyohara H, Hirano M, Wen XG, Matsumoto T, Sun XB, Yamada H (1994) Charaterisation of an anti-ulcer pectic polysaccharide from leaves of Panax ginseng C. A. Meyer. Carbohydrate Res, 263:89-101 https://doi.org/10.1016/0008-6215(94)00151-0
  18. Lee J, Lee SR (1994a) Analysis of phenolic substances contents in Korean plant foods. Korean J. Food Sci. Technol. 26:310-316
  19. Lee J, Lee SR (1994b) Some physiological activity of phenolic substances in plant foods. Korean J. Food Sci. Technol. 26:317-323
  20. Lee JW, Sohn HO, Do JH (2000) Function of the water soluble browning reaction products isolated from Korean red ginseng 2. Linoleic acid, Ox-brain autoxidant and $ Fe^{2+}$ ADP/NAD system, J. Ginseng Res. 24:35-40
  21. Miller CP, Jirkovsky I, Hayhurst DA, Adelman SJ (1996) In vitro antioxidant effects of estrogens with a hindered 3-OH function on the copper-induced oxidation of low density lipoprotein. Steroids 61:305-308 https://doi.org/10.1016/0039-128X(95)00234-H
  22. Ng TB, Wang H (2001) Panaxagin, a new protein from Chinese ginseng possesses antifungal, anti-viral, translation-inhibiting and ribonuclease activities, Life Science 68:739-749 https://doi.org/10.1016/S0024-3205(00)00970-X
  23. Nishikimi M, Rao NA, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen, Biochem. Biophys Res. Cornmun, 46:849-854 https://doi.org/10.1016/S0006-291X(72)80218-3
  24. Oliveira ACC, Perez AC, merino G, Prietp JG, Alvarez AI (2001) ProteGtive effects of Panax ginseng on muscle injury and inflammation after eccentric exercise, Comparative Biochemistry and Physiology Part C 130:369-377
  25. Park JD (1996) Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C. A. Meyer). Korean J. Ginseng Sci. 20:389-396
  26. Park. SN, Choi SW, Boo YC (1990) Effects of f1avonoids of ginseng leaves on erythrocyte membranes against singlet oxygen caused damage. Korean J. Ginseng Sci. 14:191-199
  27. Ryu GH (2003) Present status of red ginseng products and its manufacturing process. Food Industry and Nutrition 8:38-42
  28. Shin JG, Park JW, Pyo lK, Kim MS, Chung MH (1990) Protective effects of a ginseng component, maltol(2-methyl-3-hydroxyl-4-pyrone) against tissue damages induced by oxygen radicals, Korean]. Ginseng Sci. 14:187-190
  29. Sonoda Y, Ksahara T. Mukaida N, Shimizu N, Tomoda M.Takeda T (1998) Stimulation of interlukin-8 production by acidic polysaccharides from the root of Panax ginseng. Immunopharmacol. 38:287-293 https://doi.org/10.1016/S0162-3109(97)00091-X
  30. Wee JJ, Park JD, Kim MW, Lee HJ (1989) Identification of phenolic antioxidant components isolated from Panax ginseng. J. Korean Agric. Chem. Sco. 32:50-56
  31. Zhang D, yasuda T, Yu Y, Zheng P, Kawabata T, Ma Y, Okada S(1996) Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Free Radical Biology & Medicine 20:145-150 https://doi.org/10.1016/0891-5849(95)02020-9