Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Analysis of Genetic Relationships of Korean Native Polygonatum spp.

우리 나라 자생 둥굴레속 식물의 유연관계 분석

  • Jang, Kye-Hyun (Gyeongnam Agricultural Research and Extension Services) ;
  • Song, Geun-Woo (Gyeongnam Agricultural Research and Extension Services) ;
  • Chung, Jong-Il (Research Institute of Life Sci., Gyeongsang Natl. University) ;
  • Kang, Jin-Ho (Research Institute of Life Sci., Gyeongsang Natl. University)
  • Published : 2004.06.30
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Abstract

Germplasms having valuable characters are increasjngly important for modern breeding programs. This study was conducted to obtain the basic data for effective use of genetic resources of Polygonatum. The relationship of seven Polygonatum species collected widely in Korea was analyzed by RAPD markers. Total number of alleles amplified by nineteen random primers were 114 to 157, and variation in number of alleles was also diverse among seven species examined. The seven species were divided into two groups; one was of Polygonatum stenophyllum and Polygonatum humile. the other was of Polygonatum inflatum, Polygonatum lasianthum, Polygonatum odoratum var. pluriflorum (1), (2), and for variegatum.

우리 나라 전역에서 수집한 7종의 자생 둥굴레로부터 genomic DNA을 추출한 후 Operon random primer(10-mer)를 이용하여 PCR을 수행하여 재현성이 있으면서 polymorphism을 보이는 19개의 primer를 선발하였다. 선발한 primer로부터 PCR에 의해 증폭된 DNA의 크기는 $3000{\sim}300\;bp$이었고, 19개의 random primer에서 복제된 전체 band의 수는 114개에서 157개로 공시종간에 많은 차이를 보였으며, band의 유무에 근거한 유연관계 분석에서 7개의 공시종은 각시둥굴레와 층층둥굴레가 1개군으로, 여타 종들을 1개군으로 크게 2개군으로 분리되어 유전적으로 상당한 차이가 있는 것으로 나타났다.

Keywords

References

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