Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Effect of Radish on HeLa Cell Vacuolation Induced by Helicobacter pylori Cytotoxin

HeLa세포에서 Helicobacter pylori 독소에 의한 공포형성에 미치는 무의 효과

  • Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Surh, Jung-Ill (Department of Internal Medicine, College of Medicine, Dongguk University) ;
  • Chung, Yu-Jin (Department of Internal Medicine, College of Medicine, Dongguk University) ;
  • Park, In-Kyung (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Kim, Ho-Chang (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University) ;
  • Hwang, Cheorl-Weon (Department of Environmental Microbiology, Handong University) ;
  • Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 서정일 (동국대학교 의과대학 내과학교실) ;
  • 정유진 (동국대학교 의과대학 내과학교실) ;
  • 박인경 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 김호창 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 황철원 (한동대학교 생명공학부) ;
  • 김철호 (동국대학교 한의과대학 생화학분자생물학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소)
  • Published : 2004.09.30
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Abstract

Helicobacter pylori (H. pyroli) infection it associated with type B gastritis, peptic uler disease, and gastric cancer. The vacuolation of cells induced by H. pylori is thought to be essential for the initiation and maintenance of gastric infection. The roles of H. pylori cytotoxin, urease, and ammonia in the vacuolation of HeLa cells were determined. H. pylori toxin induced vacuolation of HeLa cells. Korean and Japanese radishes significantly prevented the vacuolation of HeLa cells induced by H. pylori toxin. The urease activity in vacuolated cells was also decreased with Korean and Japanese radishes. H. Pylori toxin-induced vacuolation was inhibited by vacuolar type ATPase inhibitors (bafilomycin and N-ethylmaleimide). However, further investigation is required to determine the mechanisms of radish for the inhibition of vacuole formation of eukaryotic cells in response to the H. pylori toxin.

Keywords

References

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