생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
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녹용의 품종에 따른 조혈작용 비교연구

Effect of Deer Antler Extracts from Various Origins and Parts on the Hemopoietic Activity

  • 이효정 (경희대학교 동서의학대학원 동서종양학과) ;
  • 박정란 (경희대학교 동서의학대학원 동서종양학과) ;
  • 김하나 (경희대학교 동서의학대학원 동서종양학과) ;
  • 안규석 (경희대학교 한의과대학) ;
  • 조덕연 (충남대학교 의과대학) ;
  • 최돈웅 (식품의약 안전청) ;
  • 김성훈 (경희대학교 동서의학대학원 동서종양학과)
  • Lee, Hyo-Jeong (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Park, Jeong-Ran (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim, Ha-Na (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Oriental Medical College, Kyunghee University) ;
  • Cho, Deok-Yeon (Medical College, Chungnam University) ;
  • Choi, Don-Woong (Food & Drug Administration) ;
  • Kim, Sung-Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 발행 : 2004.03.30
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초록

This study was performed to evaluate the hemopoietic effects of 6 species of deer antlers from origins and parts in vitro. CD34 positive cells were isolated and confirmed the its population by FACS analysis. In a week liquid culture, there was any statistical significance between extracts of three parts of six species of deer antlers in the experiments as colony forming assay, proliferation assay, differentiation assay and observation of morphology. However, after 2 weeks- culture with extracts of three parts of six species of deer antlers, colonies were counted. six species of deer antlers, such as middle part of Korean nippon deer, upper part of Chinese nippon deer, upper part of Newzealand horse deer, middle part of Korea horse deer and middle part of Newzealand red deer, significantly increased the CFU-GM (colony forming unit garnulocyte-macrophage) of CD34 positive cells re1atεd to production of leucocytes such as eosinophil, basophil and neutrophil, while only middle part of Korea horse deer significantly increased the BFU-E (burst forming unit-erythroid) at 1 mg/ml seggesting progenting red blood cells (RBC). In the molecular study with CD34+ cells pretreated with cyclophosphamide, antagonist of hemopoietic activity, upper Part of Korean nippon deer and upper part of Chinese nippon deer effectively increased TPO involved in a late pathway of hematopoiesis just like in ELISA assay of IL-3, TPO and GM-CSF. Taken together, these results indicate exσacts of deer antler had some hemopoietic activity still proposing more clinical study and more basic mechanism research.

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