Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibition of L-DOPA-Induced Increase in Dopamine Content by $(1R,9S)-{\beta}-Hydrastine$ Hydrochloride in PC12 cells

  • Yin, Shou-Yu (College of Pharmacy, Yanbian University) ;
  • Lee, Jae-Joon (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Yu-Mi (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Jin, Chun-Mei (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Yang, Yoo-Jung (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kang, Min-Hee (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2004.06.30
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Abstract

The effects of BHSH on L-DOPA-induced increase in dopamine content in PC12 cells were investigated. L-DOPA treatment at $20\;or\;50\;{\mu}M$ increased dopamine content after both 24 and 48 h of incubation in PC12 cells. However, the co-treatments of BHSH $(10-50\;{\mu}M)$ with L-DOPA $(20\;or\;50\;{\mu}M)$ significantly inhibited the increase of dopamine content induced by L-DOPA. BHSH treatment at $10-50\;{\mu}M$ significantly inhibited basal aromatic L-amino acid decarboxylase (AADC) activity in a concentration-dependent manner at 15 min, and then AADC activity was rapidly recovered to the control level at about 2 h. These results indicate that the inhibition of AADC activity by BHSH was, in part, contributed to the early-stage decrease of dopamine content induced by LDOPA in PC12 cells. Taken together, it is proposed that the short-term inhibition of dopamine biosynthesis by BHSH was mediated by the regulation of tyrosine hydroxylace (TH).

Keywords

References

  1. Baroni, A., Benvenuti, F., Fantini, L., Pantaleo, T., and Urbani, F. (1984) human ballistic ann abduction movements: Effects of L-DOPA treatment in Parkinson's disease. Neurology. 34, 868-876 https://doi.org/10.1212/WNL.34.7.868
  2. Byrd, J.C., Hadjiconstantinou, M., and Cavalla, D. (1986) Epinephrine synthesis in the PC12 pheochromocytoma cell line. Eur. J. Pharmacol. 127, 139-142 https://doi.org/10.1016/0014-2999(86)90216-5
  3. Chang, W.Y. and Webster, R.A. (1997) Effect of L-dopa alone and with benserazide on the spontaneous activity of striatal neurons in normal and 6-hydroxydopamine-lesioned rats. Br. J. Pharmacol. 121, 331-337 https://doi.org/10.1038/sj.bjp.0701133
  4. Chase, T.N., Baronti, F, Fabbrini, G., Heuser, I.J., Juncos, J.L., and Mouradian, M.M. (1989) Rationale for continuous dipaimnomimetic therapy of Parkinson's desease. Neurology. 39, 7-10
  5. Chase, T.N., Engber, T.M., and Mouradian, M.M. (1994) Palliative and prophylactic benefits of continuously administered dopaminomimetics in Parkinson's disease. Neurology. 44, S15-S18
  6. Deitrich, R. and Erwin, V. (1980) Biogenic amine aldehyde condensation products: Tetrahydroisoquinolines and tryptolines ($\beta$-carbolines). Ann. Rev. Pharmacol. Toxicol. 20, 55-80 https://doi.org/10.1146/annurev.pa.20.040180.000415
  7. Rommelspacher, H., May, T., and Susilo, R. (1991) $\beta$-Carbolines and tetrahydroisoquinolines: Detecdon and function in mammals. Planta Med. 57 (Suppl), S85-S92 https://doi.org/10.1055/s-2006-960234
  8. Myers, R.D. (1989) Isoquinolines, beta-carbolines and alcohol drinking: Involvement of opioid and dopaminergic mechanisms. Experientia. 45, 436-443 https://doi.org/10.1007/BF01952025
  9. Fabbrini, G., Mouradian, M.M., Juncos, j.L., Schlegel, J., Mohr, E., and Chase, T.N. (1988) Motor fluctuations in Parkinson's disease: central pathophysiological mechanisms, Prt J. Ann. Neurol. 24, 366-371 https://doi.org/10.1002/ana.410240303
  10. Fang, Q. C., Lin, M., Weng, Q., Zhu, C., and Liu, X. (1981) Chemistry of alkaloids of Corydalis--chemical studies on alkaloids of four Corydalis species from Qinghai plateau (China).Yaoxue Xuebao. 16, 798-800
  11. Gebreyesus, K., Kilbourne, E.J., and Sabban, E.L. (1993) Bradykinin elevates tyrosine hydroxylase and dopamine $\alpha$-hydroxylase mRNA in PC12 cells. Brain Res. 608, 345-348 https://doi.org/10.1016/0006-8993(93)91477-A
  12. Gjedde, A., Leger, G.C., Cumming, P, Yasuhara, Y., Evans, A.C., Guttman, M., and Kuwabara, H. (1993) Striatal L-dopa decarboxylase activity in Parkinson's desease in vivo: implications for the regulation of dopamine synthesis. J. Neurochem. 61, 1538-1541 https://doi.org/10.1111/j.1471-4159.1993.tb13651.x
  13. Hefti, F., Melamed, E., and Wurtman, R.J. (1980) The decarboxylation of DOPA in the parkinsonian brain: in vivo studies of an animal model. J. Neural Transm. Suppl. 16, 95-101
  14. Hefti, F, Melamed, E., and Wurtman, R.J. (1981) The site of dopamine formation in rat striatum after L-dopa administration. J. Pharmacol. Exp. Ther. 217, 189-197
  15. Hughes, A.J., Damiel, S.E., Blankson, S., and Lees, A.J. (1993) A clinicopathologic study of 100 cases of Parkinson's disease. Arch. Neurol. 50, 140-148 https://doi.org/10.1001/archneur.1993.00540020018011
  16. Kim, S.H., Shin, J.S., Lee, J.J., Yin, S.Y., Kai, M., and Lee, M.K. (2001) Effects of hydrastine derivatives on dopamine biosynthesis in PC12 cells. Planta Med. 67, 609-613 https://doi.org/10.1055/s-2001-17356
  17. Kordower, J.H., Goetz, C.G., Freeman, T.B., and Olanow, C.W. (1997) Dopaminergic transplants in patients with Parkinson's disease: Neuroanatomical correlates of clinical recovery. Exp. Neurol. 144, 41-46 https://doi.org/10.1006/exnr.1996.6386
  18. Langston, J.W., Ballard, R., Tetrud, J.W., and Irwin, I. (1983) Chronic parkinsonism in humans due to a product of meperidine-analog synthesis. Science. 219, 979-980 https://doi.org/10.1126/science.6823561
  19. Lee, M.K. and Kim, H.S. (1996) Inhibitory effects of protoberberine alkaloids from the root of Coptic japonica on catecholamine biosynthesis in PC12 cell. Planta Med. 62, 31-34 https://doi.org/10.1055/s-2006-957791
  20. Lloyd, K. and Hornykiewicz, O. (1970) Parkinson's desease: activity of L-DOPA decarboxylase in descrete brain regions. Science. 170, 1212-1213 https://doi.org/10.1126/science.170.3963.1212
  21. Melamed, E. and Hefti, F. (1984) Mechaniam of action of short-and long-term L-DOPA treatment in Parkinsonism: role of the surviving nigrostriatal dopaminergic neurons. Adv. Neurol. 40, 149-157
  22. Melamed, E., Hefti, F., and Wurtman, R.J. (1979) Nonaminergic striatal neurons convert exogenous L-DOPA to dopamine in Parkinsonism. Ann. Neurol. 8, 558-563
  23. Melamed, E., Hefti, F., and Wurtman, R.J. (1980) Decarboxylation of exogenous L-DOPA in rat striatum after lesions of the dopaminergic nigrostriatal neurons: the role of striatal capillaries Brain Res. 198, 244-248 https://doi.org/10.1016/0006-8993(80)90363-7
  24. Neff, N.H. and Hadjiconstantinou, M. (1995) Aromatic L-amino acid decarboxylase modulation and Parkinson's desease. Prog. Brain Res. 106, 91-97 https://doi.org/10.1016/S0079-6123(08)61206-6
  25. Rogers, M.W. and Chan, C.W.Y. (1988) Motor planning is impaired in Parkinsons desease. Brain Res. 438, 271-276 https://doi.org/10.1016/0006-8993(88)91346-7
  26. Schallert, T., Jones, T.A., Norton, D. (1992) A clinically relevant unilateral rat model of Parkinsonian akinesia. J. Neural transplant Plast. 3, 332-333 https://doi.org/10.1155/NP.1992.332
  27. Shin, J. S., Kim, K.T., and Lee, M.K. (1998a). Inhibitory effects of bulbocapnine on dopamine biosynthesis in PC12 cells. Neurosci. Lett. 244, 161-164 https://doi.org/10.1016/S0304-3940(98)00148-7
  28. Shin, J.S., Kim, E.I., Kai, M., Kim, H.S., and Lee, M.K. (1998b) Inhibitory effects of coralyne on dopamine content in PC12 cells. Med. Sci. Res. 26, 745-747
  29. Shin, J.S., Yun-Choi, H.S., Kim, E.I., and Lee, M.K. (1999) Inhibitory effects of higenamine on dopamine content in PC12 cells. Planta Med. 65, 452-455 https://doi.org/10.1055/s-2006-960810
  30. Stocchi, F, Bonamartini, A., Vacca, L., and Ruggieri, S. (1996) Motor fluctuations in levodopa treatment: Clinical pharmacology. Eur. Neurol. 36, 38-42 https://doi.org/10.1159/000118882
  31. Thull, U., Kneubuhler, S., Gaillard, P., Carrupt, P.A., Testa, B., Altomare, C., Carotti, A., Jenner, P., and McNaught, KStP. (1995) Inhibition of monoamine oxidase by isoquinoline derivatives. Biochem. Pharmacol. 50. 869-877 https://doi.org/10.1016/0006-2952(95)00220-T
  32. Tischler, A.S., Perlman, R.L., Morse, G.M., and Sheard, B.E. (1983) Glucocorticoids increase catecholamine synthesis and storage in PC12 pheochromocytoma cell culture. J. Neurochem. 40, 364-370 https://doi.org/10.1111/j.1471-4159.1983.tb11291.x
  33. Zhu, M.Y. and Juorio, A.V. (1995) Aromatic L-amino acid decarboxylase: biological characterization and functional role Gen. Pharmacol. 26, 681-696