Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Apoptosis-Inducing Activity of Galloylglucoses from Juglans mandshurica in Human Promyeloid Leukemic HL-60 Cells

  • Min, Byung-Sun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Ok-Kyoung (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Bo-Young (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Hattori, Masao (Institute of Natural Medicine, Toyama Medical and Pharmaceutical University) ;
  • Joung, Hyouk (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Hyeong-Kyu (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2004.02.28
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Abstract

Two galloyl monosaccharides, 1,2,6-trigalloylglucose (1, TRgG) and 1,2,3,6- tetragalloylglucose (2, TEgG), were isolated from the stem-bark of Juglans mandshurica. Two galloylglucoses showed cytotoxic effects on human promyelocytic leukemia HL-60 cells. In order to elucidate their mechanism of action, we have investigated the flow cytometric analysis after Annexin V-FITC and PI staining, caspase-3 activity, and internucleosomal DNA fragmentation in HL-60 cells. HL-60 cells treated with both compounds 1 and 2 at 150 and $100\;{\mu}M$, respectively, led to a morphological features of apoptosis, such as plasma membrane blebbing and cell shrinkage. TRgG (1) and TEgG (2) increased the percentage of $FITC^+\;and\;FITC^+PI^+$ cells in flow cytometry after Annexin V-FITC and PI staining. The increase of apoptotic cells was preceded by the activation of caspase-3 reported to play a central role in apoptotic process and inducing internucleosomal DNA fragmentation. TEgG (2) showed to have stronger apoptosis inducing activity in HL-60 cell lines as compared with TRgG (1).

Keywords

References

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