동의신경정신과학회지 (Journal of Oriental Neuropsychiatry)

  • 제15권1호
  • /
  • Pages.77-86
  • /
  • 2004
  • /
  • 1226-6396(pISSN)
  • /
  • 2234-4942(eISSN)
대한한방신경정신과학회 (The Korean Society of Oriental Neuropsychiatry)
권호 표지

천마전헐탕의 $GABA_A/benzodiazepine$ 신경수용체(神經受容體) 효능활성(效能活性)에 관(關)한 연구(硏究)

Study on the Agonistic Effect of Chunmajeongal-tang Extract to the $GABA_A/benzodiazepine$ Receptor Complex

  • 김성욱 (동국대학교 한의과대학 신경정신과학교실) ;
  • 공대종 (동국대학교 한의과대학 신경정신과학교실) ;
  • 안현국 (동국대학교 한의과대학 신경정신과학교실) ;
  • 김근우 (동국대학교 한의과대학 신경정신과학교실) ;
  • 구병수 (동국대학교 한의과대학 신경정신과학교실)
  • Kim, Sung-Wook (Dept. of Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Gong, Dae-Jong (Dept. of Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • An, Hyeon-Guk (Dept. of Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Kim, Geun-Woo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dongguk University) ;
  • Koo, Byung-Soo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dongguk University)
  • 발행 : 2004.06.30
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초록

Objective : This study was performed to investigate the agonistic activity of Chunmajeongal-tang extract to the $GABA_A/benzodiazepine$ receptor complex. Methods : Male mice and Sprague-Dawley rats were used for this experiment. Chunmajeongal-tang Prescription was extracted with 80% methanol, evaporated in vacuo and dried with freeze dryer. The agonistic activity to the GABA/ benzodiazepine receptor complex and GABA transaminase activity were measured in vitro. Results : Chunmajeongal-tang extract inhibited dose-dependently the binding of [3H]Ro15-1788, an antagonist on GABA/benzodiazepine receptor complex, in rat cerebral cortices, showing $82.4{\pm}4.12%$ inhibition at a dose of 5.0 mg/kg. This extract inhibited dose-dependently the binding of [3H]flunitrazepam, an agonist on GABA/benzodiazepine receptor complex, in rat cerebral cortices, showing $5.6{\pm}1.24%$ inhibition. Furthermore, Chunmajeongal-tang extract inhibited the binding of [3H]flunitrazepam in the presence of GABA/NaCI with $13.2{\pm}0.44%$ inhibition, its inhibitory effect exhibited a positive GABA shift, which means that this extract activates a GABAergic neurotransmission.

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