The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지

Effect of velvet antler on the function of adipocytes(3T3-L1) and its association with IGF-1

녹용(鹿茸)이 지방세포(脂肪細胞)(3T3-L1)와 성장인자(成長因子)에 미치는 영향(影響)

  • Jun, Chan-Il (Department of Pediatrics, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Jin-Yong (Department of Pediatrics, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Deog-Gon (Department of Pediatrics, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ki-Hoon (Department of Pediatrics, College of Oriental Medicine, Kyung Hee University)
  • 전찬일 (경희대학교 한의과대학 소아과학교실) ;
  • 이진용 (경희대학교 한의과대학 소아과학교실) ;
  • 김덕곤 (경희대학교 한의과대학 소아과학교실) ;
  • 김기훈 (경희대학교 한의과대학 소아과학교실)
  • Published : 2004.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: These experimental studies were designed to investigate the effects of antler's extracts on the expression of leptin, IGF-l and IGF-1 receptor on cultured 3T3-L1 cells. Methods: A mouse adipoblast cell line, 3T3-L1, was cultured with or without a differentiation medium containing Isobutylmethylxanthin, Dexametasone and Insulin before adding extracts of antler of various concentratio(10, 50, ]00, 200${\mu}g/ml$). The expression of leptin and IGF-1 receptor was measured by western blot assay, and expression of IGF-1 was determined by F ACS analysis. Results and Conclusion: The 3T3-L1 cells' differentiation did not show a significant induction by extracts of antler. The expression of leptin was significantly decreased depend on antler's concentration. The expression of IGF-1 showed a slightly increase by extracts of antler, whereas that of IGF-receptor showed a tendency to increase. The total amount of intracellular triglyceride showed a tendency to diminish as the concentration of antler's extract increase.

Keywords