The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지

Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

  • Lee, Young-Suk (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Kim, Na-Ri (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Kim, Hyun-Ju (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Joo, Hyun (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Kim, Young-Nam (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Jeong, Dae-Hoon (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Cuong, Dang Van (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Kim, Eui-Yong (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Hur, Dae-Young (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Park, Young-Shik (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Hong, Yong-Geun (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Lee, Sang-Kyung (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Chung, Joon-Yong (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Seog, Dae-Hyun (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University) ;
  • Han, Jin (2020 Cardiovascular Institute, Department of Physiology and Biophysics, College of Medicine, Inje University)
  • Published : 2004.08.21
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and subjected to trypsin digestion, followed by MALDI-MS. The protein was identified as myoglobin, which was confirmed also by Western blot analysis. These results are consistent with previous studies of cardiac markers in ischemic hearts, indicating myoglobin as a suitable marker of myocardial injury. In addition, the present use of multiple techniques indicates that proteomic analysis is an appropriate means to identify cardiac markers in studies of IPC and IR.

Keywords

References

  1. Agnillo FD, Alayash AI. A role of the myoglobin redox cycle in the induction of endothelial cell apoptosis. Free Radical Biology & Medicine 33: 1153-1164, 2002 https://doi.org/10.1016/S0891-5849(02)01007-9
  2. Alaya AI, Patel RP, Cashon RE. Redox reactions of hemoglobin and myoglobin: biological and toxicological implications. Antioxid Redox Signal 3: 313-327, 2001 https://doi.org/10.1089/152308601300185250
  3. Alderton AL, Faustman C, Liebler DC, Holl DW. Induction of redox instability of bovine myoglobin by adduction with 4-hydroxy- 2-nonenal. Biochemistry 42: 4398-4405, 2003 https://doi.org/10.1021/bi0271695
  4. Antonini E, Brunori M. Hemoglobin and myoglobin in their reactions with ligands. Elsevier, New York, 1971
  5. Braunwald EK. Myocardial reperfusion: a double- edged sword? J Clin Invest 76:1713-1719, 1985 https://doi.org/10.1172/JCI112160
  6. Carroll R, Walker JM, Yellon DM. Myocardial protection: The role of the KATP channel. J Clin Basic Cardiol 2: 8, 1999
  7. Charlwood J, Skehel FM, camilleri P. Analysis of N-linked oligosaccharides released from glycoproteins separated by two-dimensional gel electrophoresis. Anal Biochem. 284: 49-59, 2000 https://doi.org/10.1006/abio.2000.4687
  8. Christian WH. Cardiac biomarkers for rapid evaluation of chest pain. Circulation 104: 1454-1456, 2001
  9. Cohen MW, Baines CP, Downey JM. Ischemic preconditioning: from adenosine receptor to KATP channel. Annu Rev Physiol 62:79- 109, 2000 https://doi.org/10.1146/annurev.physiol.62.1.79
  10. Drexel H, Dworzak E, Kirchmair W. Myoglobinemia in the early phase of acute myocardial infarction. Am Heart J 105: 642-651, 1983 https://doi.org/10.1016/0002-8703(83)90489-1
  11. Esquerra RM, Goldbeck RA, Kim-shapiro DB, Kliger DS. Spectroscopic evidence for nanosecond protein relaxation after photodissociation of myoglobin-CO. Biochemistry 37: 17527-17536, 1998 https://doi.org/10.1021/bi9814437
  12. Ferdinandy P, Schulz R. Nitric oxide, superoxide, and peroxynitrite in myocardial ischemia-reperfusion injury and preconditioning. British Journal of Pharmacology 138:532-543, 2003 https://doi.org/10.1038/sj.bjp.0705080
  13. Galaris D, Eddy L, Arduini A, Cadenas E, Hochstein P. Mechanisms of reoxygenation injury in myocardial infarction implications of a myoglobin redox cycle. Biochem Biophys Res Commun 160: 1162-1168, 1989 https://doi.org/10.1016/S0006-291X(89)80125-1
  14. Grenadier E, Keidar S, Kahana L. The role of serum myoglobin, total KCP and CK-MB isoenzyme in the acute phase of myocardial infarction. Am Heart J 105: 408-416, 1983 https://doi.org/10.1016/0002-8703(83)90357-5
  15. Holt S, Moore K. Pathogenesis of renal failure in rhabdomyolysis: the role of myoglobin. Exp Nephrol 8: 72-76, 2000 https://doi.org/10.1159/000020651
  16. Hudson MP, Christenson RH, Newby LK, Kaplan AL, Ohman EM. Cardiac markers: point of care testing. Clinica Chimica Acta 284: 223-237, 1999 https://doi.org/10.1016/S0009-8981(99)00083-2
  17. Jannie W, Felocotas LL, Robert RS, David L, John BM. Is myoglobin useful in the diagnosis of acute myocardial infarction in the emergency department setting? Am J Clin Phathol 103: 725-729, 1995
  18. Jannie W, Felocotas LL, Robert RS, David L, John BM. Is myoglobin useful in the diagnosis of acute myocardial infarction in the emergency department setting? Am J Clin Phathol 103: 725-729, 1995
  19. Katrin M, Dorian I, Joachim S, Helmut EM. Identification of platelet proteins separated by two-dimensional gel electrophoresis and analyzed by matrix-assisted laser desorption/ ionization-time tyrosine-phosphorylated proteins. Electrophoresis 21: 2622-2636, 2000 https://doi.org/10.1002/1522-2683(20000701)21:13<2622::AID-ELPS2622>3.0.CO;2-3
  20. Klocke F, Copley DP, Krawczyk A, Leichlin M. Rapid renal clearance of immunoreactive canine plasma myoglobin. Circulation 65: 1522-1528, 1982 https://doi.org/10.1161/01.CIR.65.7.1522
  21. Larsson T, Bergstrom J, Nilsson C, Karlsson KA. Use of an affinity proteomics approach for the identification of low-abundant bacterial adhesions as applied on the Lewis (b)-binding adhesin of Helicobacter phlori. FEBS Lett 469: 155-158, 2000 https://doi.org/10.1016/S0014-5793(00)01270-9
  22. Li Y, Whittaker P, Kloner RA. The transient nature of the effect of ischemic preconditioning on myocardial infarct size and ventricular arrhythmias. Am Heart J 23:346-353, 1992
  23. Mark PM, Ben RH, Martin BS, Thierry R, Amanda SN, Keith LW, Andrew AG. Proteomic analysis of Escherichia coli outer membrane. Eur J Biochem 267: 2871-2881, 2000 https://doi.org/10.1046/j.1432-1327.2000.01296.x
  24. Michael PH, Robert HC, Newby LK, Andrew LK, Ohman EM. Cardiac markers: point of care testing. Clinica Chimica Acta 284: 223-237, 1999 https://doi.org/10.1016/S0009-8981(99)00083-2
  25. Moore KP, Holt SG, Patel RP, Svistunenko DA, Zackert W, Goodier D, Reeder BJ, Clozel M, Anand R, Cooper CE, Morrow JD, Wilson MT, Darley-Usmar V, Roberts LJ. A causative role for redox cycling of myoglobin and its inhibition by alkalinization in the pathogenesis and treatment of rhabdomyolisis-induced renal failure. J Biol Chem 273: 31731-31737, 1998 https://doi.org/10.1074/jbc.273.48.31731
  26. Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: A delay of lethal cell injury in ischemic myocardium. Circulation 75:1124-1136, 1986
  27. Park KS, Kim HG, Kim NG, Cho AY, Choi KH, Seong JK, Paik YK. Proteomic analysis and molecular characterization of tissue ferritin light chain in hepatocellular carcinoma. Hepatology 35: 1459-1466, 2002 https://doi.org/10.1053/jhep.2002.33204
  28. Penttil K, Koukkunen H, Kemppainen A, Halinen M, Rantanen T, Pyrl K, Penttil I. Myoglobin, creatine kinase MB, troponin T, and troponin I- rapid bedside assays in patients with acute chest pain. Int J Clin Lab Res 29: 93-101, 1999
  29. Przklenk K, Kloner RA. Ischemic preconditioning: Exploring the paradox. Pro Cardiovasc Dis 40: 516-547, 1998
  30. Robert AK, Thomas S, Chrisyopher PC, and Karin P. Ischemic preconditioning: Implications for the geriatric heart. AJGC 10: 145-151, 2001 https://doi.org/10.1111/j.1076-7460.2001.00002.x
  31. Robert AK, Thomas S, Chrisyopher PC, Karin P. Ischemic preconditioning: Implications for the geriatric heart. AJGC 10:145- 151, 2001 https://doi.org/10.1111/j.1076-7460.2001.00002.x
  32. Ryu JW, Kim HJ, Lee YS, Myong NH, Hwang CH, LeeGS, Yom HC. The proteomics approach to find biomarkers in gastric cancer. J Korean Med Sci 18:505-509, 2003
  33. Srinivas PR, Kramer BS, Srivastava S. Trends in biomarker research for cancer detection. Lancet Oncol 2: 698-704, 2001 https://doi.org/10.1016/S1470-2045(01)00560-5
  34. Svensson L, Axlsson C, Nordlander R, Herlitz J. Elevation of biochemical markers of myocardial damage prior to hospital admission in patients with acute chest pain or other symptoms raising suspicion of acute coronary syndrome. Journal of Internal Medicine 253: 311-319, 2003 https://doi.org/10.1046/j.1365-2796.2003.01116.x
  35. Wilkins A, Mann M. Proteomics to study genes and genomes. Nature 405: 837-846, 2000 https://doi.org/10.1038/35015709
  36. Wilkins MR, Sanchez JC, Gooley AA, Appel RD, Humphery-Smith L, Hochstrasser DF, Williams KL. Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Gen Eng Rev 13: 19-50, 1996
  37. Wittenberg BA, Wittenberg JB. Transport of oxygen in muscle. Annu Rev Physiol 57: 857-878, 1989
  38. Yellon DM, Bexter GF. A 'second window of protection' or delayed preconditioning phenomenon: Future horizons for myocardial protection. J Mol Cell Cardiol 27: 1023-1034, 1995 https://doi.org/10.1016/0022-2828(95)90071-3