The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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The Effect of Ghrelin on $Ca^{2+}$ Concentration in Thyroid FRTL-5 Cells

  • Kim, Byung-Joo (Department of Physiology and Biophysics, Seoul National University College of Medicine, and Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Park, Young-Joo (Bundang Seoul National University Hospital) ;
  • Park, Do-Joon (Seoul National University Hospital) ;
  • So, In-Suk (Department of Physiology and Biophysics, Seoul National University College of Medicine, and Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Kim, Ki-Whan (Department of Physiology and Biophysics, Seoul National University College of Medicine,Department of Internal Medicine, Seoul National University College of Medicine)
  • Published : 2004.08.21
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Abstract

Ghrelin is a newly discovered peptide, which is released from the stomach and neurons in the hypothalamic arcuate nucleus (ARC), and potently stimulates growth hormone release and food intake. In the present study, we investigated the effect of ghrelin on $[Ca^{2+}]_i$ in thyroid FRTL-5 cells. Ghrelin (5 nM) increased $[Ca^{2+}]_i$ and TSH (1 unit/l) had an additive effect on $[Ca^{2+}]_i$ when extracellular normal solution was 1.1mM $Ca^{2+}$ containing Coon's modified Ham's F12 medium. When $Ca^{2+}-free$ medium containing 2 mM EGTA replaced the above normal solution, ghrelin also induced a similar rise in $[Ca^{2+}]_i$. In the middle of $[Ca^{2+}]_i$ increment by ghrelin, nifedipine $(1\;{\mu}M)$, nickel $(100\;{\mu}M)$ and $La^{3+}\;(100\;{\mu}M)$ had no effect on $[Ca^{2+}]_i$. After endoplasmic reticulum was depleted by cyclopiazonic acid $(CPA;10\;{\mu}M)$, ghrelin caused no visible change on $[Ca^{2+}]_i$ in $Ca^{2+}-free$/2 mM EGTA solution. These results suggest that ghrelin can increase $[Ca^{2+}]_i$ through endoplasmic reticulum in thyroid FRTL-5 cells.

Keywords

References

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