대한한방내과학회지 (The Journal of Internal Korean Medicine)
- 제25권4호
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- Pages.227-241
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- 2004
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- 1226-9174(pISSN)
시호(柴胡)의 뇌해마 신경세포 보호효능에 대한 연구
A Study of Neuroproctective Effect of Bupleuri Radix on Hippocampal Neurons
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Lee, Won-Chul
(Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
- Shin, Kwang-Sik (Department of Internal Medicine, College of Oriental Medicine, Dongguk University)
- 발행 : 2004.12.30
초록
Objective : This study was performed to investigate neuroprotective effects of Bupleuri Radix against oxidative and ischemic damages. Method : To observe the neuroprotective effects against ischemic damage, ischemic insult was induced by oxygen/glucose deprivation (OGD) on organotypic hippocampal slice cultures (OHSC) from 1 week-old Sprague-Dawley rats. Propidium iodide (PI) fluorescence-stained neuronal dead-cell areas, area percentages and TUNEL-positive apoptotic cells in CA1 and dentate gyrus, and LDH levels in culture media of the OHSC were measured following Bupleuri Radix extract treatment. Result : The following results were obtained: (1) The