Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

The Protective Effect of Phospholipase $A_2(PLA_2)$ Herbal-acupuncture against the Neuronal Damage Induced by Middle Cerebral Artery Occulsion(MCAO) in Rats.

Phospholipase $A_2(PLA_2)$ 약침(藥鍼)이 중대뇌동맥폐색(中大腦動脈閉塞)으로 유발(誘發)된 흰쥐의 신경손상(神經損傷) 보호(保護) 효과(效果)에 미치는 영향

  • Kim, Sung-Min (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, DaeguHanny University) ;
  • Jung, Tae-Young (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, DaeguHanny University) ;
  • Leem, Seong-Cheol (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, DaeguHanny University) ;
  • Seo, Jeong-Chul (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, DaeguHanny University) ;
  • Kim, Mi-Ryeo (Dept. of Physiology, College of Oriental Medicine, DaeguHanny University) ;
  • Yang, Chae-Ha (Dept. of Pharmacology, College of Oriental Medicine, DaeguHanny University) ;
  • Han, Sang-Won (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, DaeguHanny University)
  • 김성민 (대구한의대학교 한의과대학 침구학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구학교실) ;
  • 김미려 (대구한의대학교 한의과대학 생리학교실) ;
  • 양재하 (대구한의대학교 한의과대학 약리학교실) ;
  • 한상원 (대구한의대학교 한의과대학 침구학교실)
  • Published : 2004.09.27
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Abstract

Objectives : In order to prove the effect of Phospholipase $A_2(PLA_2)$ Herbal-acupuncture, this experimental studies were performed by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). Methods : Microdialysis probes were implanted into the coordinate of striatum of anesthetized rats which consist of sham-operated 8 rats, MCAO-operated 8 rats and $PLA_2$ Herbal-acupuncture administrated 8 rats before MCAO operating. The $PLA_2$ Herbal-acupuncture(0.5mg/kg) was administrated to rats 30 minutes before having an operation causing the MCAO. The surgical excision lead the cross resected brain to the acute ischemic state. The brain was sliced in 2mm thickness and stained with cresyl violet buffer for the measurement of cerebral infarcted area and volume. Results : Based on the result of the tissue inspection for the cerebral ischemic cell, $PLA_2$ Herbal-acupuncture significantly protect neurocytes. Conclusions : We suggest $PLA_2$ Herbal-acupuncture produces protective effects against the neuronal damage induced by MCAO. Therefore, $PLA_2$ Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

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