동충하초(冬蟲夏草) 약침(藥鍼)이 백서(白鼠)의 기관지평활근(氣管支平滑筋) 장력(張力)과 염증(炎症) 및 면역(免疫) 반응(反應)에 미치는 영향(影響)

Effects of Cordyceps militaris Mycelia(CMM) herbal acupuncture at BL13, LU4 on airway smooth muscle, airway inflammation, IgE and Interleukin-4 in mouse model of allergic bronchial asthma

  • 윤대환 (동신대학교 한의과대학 경혈학교실) ;
  • 최관준 (동신대학교 한의과대학 침구학교실) ;
  • 채우석 (동신대학교 한의과대학 침구학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈학교실)
  • Youn, Dae-Hwan (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Choi, Gwan-Jun (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Chae, Woo-Seok (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Na, Chang-Su (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dongshin University)
  • 발행 : 2004.06.27

초록

Objectives : It has been reported that Cordyceps militaris Mycelia(CMM) has an effect on deficiency allergic asthma(虛喘) clinically. The aim of this study was to determine an appropriate oriental treatment and the doses of CMM to treat asthma. Methods : In order to study the effect of herbal acupuncture solution of the CMM on allergic asthma, the mouse were pretreated by CMM herbal acupuncture at BL13, LU4 before antigen sensitization. 2 days later Mice were actively sensitized with a subcutaneous injection of ovalbumin(OA) and 13 days later they were provoked with OA aerosols. IL-4, lymphocyte, macrophage in bronchoalveolar lavage fluid(BALF), IgE in serum, WBC, RBC, HGB in blood, and in vitro isometric contractile responses of the isolated tracheal smooth muscle(TSM) to acetylcholine$(ACh,\;0.1-1000\;{\mu}M)$, KCl were measured. Results : Contractile responses of TSM to ACh were significantly increased in CMM herbal acupuncture 1 group $(Ach\;1000\;{\mu}M)$, CMM herbal acupuncture 2 group $(ACh\;1,\;10\;{\mu}M)$, CMM herbal acupuncture 3 group $(Ach\;0.3,\;1,\;30,\;300\;{\mu}M)$. The sensitivity of TSM to ACh was significantly decreased in CMM herbal acupuncture 3 group. The maximal contractile response of TSM to ACh was significantly decreased in CMM herbal acupuncture 1, 3 group. The maximal contractile response of TSM to KCl was significantly decreased in CMM herbal acupuncture 1, 2, 3 group. The counts of lymphocytes in BALF was significantly increased in CMM herbal acupuncture 3 group. The counts of macrophages in BALF was significantly decreased in CMM herbal acupuncture 3 group. Interleukin-4 level in BALF was significantly increased in CMM herbal acupuncture 1,3 group. and it was increased in CMM herbal acupuncture 2 group, but there was no significance. Serum IgE level was significantly decreased in CMM herbal acupuncture 1, 2, 3 group. The counts of WBC in blood was significantly increased in CMM herbal acupuncture 1, 3 group Conclusion : Based on the above results it is assumed that CMM herbal acupuncture at BL13, LU4 can help the treatment of deficiency allergic Asthm.

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