Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Electrochemical and Spectroscopic Studies on the Interaction between DNA and the Product of Enzyme-catalyzed Reaction of OPD-H2O2-HRP

  • Niu, S.Y. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology) ;
  • Zhang, S.S. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology) ;
  • Ma, L.B. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology) ;
  • Jiao, K. (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology)
  • Published : 2004.06.20
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Abstract

The pure product of 2,3-diaminophenazine was prepared by the enzyme-catalyzed reaction of ophenylenediamine-$H_2O_2$-horseradish peroxidase and characterized by UV/Vis spectroscopy, IR spectroscopy and NMR spectroscopy. The electrochemical behaviour of 2,3-diaminophenazine on the glassy carbon electrode was studied. The interaction between 2,3-diaminophenazine and deoxyribonucleic acid was studied by cyclic voltammetry method and UV/Vis spectroscopy, which indicated that the interaction between them is intercalation. The influence of reacting time was also studied. The binding ratio of the 2,3-diaminophenazine-DNA complex is calculated to be 1 : 2 and the binding constant is to be $5.07{\times} 10^3L{\cdot}mol^{-1}$ at room temperature.

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