Bulletin of the Korean Chemical Society

  • 제25권9호
  • /
  • Pages.1366-1370
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  • 2004
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Effect of Linker for Immobilization of Glutathione on BSA-Assembled Controlled Pore Glass Beads

  • Chen, Li-Hua (Center for Integrated Molecular Systems, Department of Chemistry, Pohang University of Science and Technology, Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University) ;
  • Choi, Young-Seo (Center for Integrated Molecular Systems, Department of Chemistry, Pohang University of Science and Technology) ;
  • Park, Jung-Won (Center for Integrated Molecular Systems, Department of Chemistry, Pohang University of Science and Technology) ;
  • Kwon, Joseph (Sigmol Incorporation) ;
  • Wang, Rong-Shun (Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University) ;
  • Lee, Tae-Hoon (Sigmol Incorporation) ;
  • Ryu, Sung-Ho (Department of Life Science, Pohang University of Science and Technology) ;
  • Park, Joon-Won (Center for Integrated Molecular Systems, Department of Chemistry, Pohang University of Science and Technology)
  • 발행 : 2004.09.20
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초록

Controlled pore glass bead was modified with bovine serum albumin (BSA), and glutathione (GSH) was immobilized through three kinds of linkers on top of BSA. Bis(3-sulfo-N-hydroxysuccinimide suberate) sodium salt $(BS^3)$, N-hydroxysuccinimide 3-(2-pyridyldithio)propionate (SPDP), or N-hydroxysuccinimide 4-maleimidobutyrate (GMBS) was introduced into the BSA-bound matrix. Subsequently, GSH was immobilized by addition of thiol side chain into the maleimido moiety, replacing a disulfide group, or formation of an amide group upon releasing 3-sulfo-N-hydroxysuccimide group. It was observed that conjugation methodology played a critical role for activity of the immobilized GSH. SDS-PAGE chromatogram showed that the matrix of glutathione immobilized on BSA through GMBS manifested high selectivity towards glutathione-S-transferase (GST) in cell lysate.

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참고문헌

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