Bulletin of the Korean Chemical Society

  • 제25권12호
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  • Pages.1833-1839
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  • 2004
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Development of Ultra-High Pressure Capillary Reverse-Phase Liquid Chromatography/Tandem Mass Spectrometry for High-Sensitive and High-Throughput Proteomics

  • Kim, Min-Sik (Department of Chemistry and Center for Electro- & Photo-Responsive Molecules, Korea University) ;
  • Choie, Woo-Suk (Department of Chemistry and Center for Electro- & Photo-Responsive Molecules, Korea University) ;
  • Shin, Yong-Seung (Functional Proteomics Center, Korea Institute of Science and Technology, School of Life Sciences, Korea University) ;
  • Yu, Myeong-Hee (Functional Proteomics Center, Korea Institute of Science and Technology) ;
  • Lee, Sang-Won (Department of Chemistry and Center for Electro- & Photo-Responsive Molecules, Korea University)
  • 발행 : 2004.12.20
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초록

Recently mass spectrometry and separation methods such as liquid chromatography have become major tools in the field of proteomics. In this report, we describe in detail our efforts to develop ultra-high pressure capillary reverse-phase liquid chromatography (cRPLC) and its online coupling to a mass spectrometer by a nanoelectrospray (nanoESI) interface. The RPLC system is constructed in house to deliver LC solvents at the pressure up to 20,000 psig, which is four times higher than conventional RPLC systems. The high operation pressure allows the efficient use of packed micro-capillary columns (50, 75 and 150 ${\mu}$m i.d., up to 1.5 m long). We will discuss the effect of column diameter on the sensitivity of cRPLC/MS/MS experiments and the utility of the developed technique for proteome analysis by its application in the analysis of proteome samples having different levels of complexity.

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참고문헌

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피인용 문헌

  1. Postexperiment Monoisotopic Mass Filtering and Refinement (PE-MMR) of Tandem Mass Spectrometric Data Increases Accuracy of Peptide Identification in LC/MS/MS vol.7, pp.6, 2008, https://doi.org/10.1074/mcp.M700419-MCP200
  2. Capillary Liquid Chromatography at Ultrahigh Pressures vol.3, pp.1, 2010, https://doi.org/10.1146/annurev.anchem.1.031207.113014
  3. The effect and potential of using a temperature controlled separation column with ultra-high pressure microcapillary liquid chromatography/tandem mass spectrometry on proteomic analysis vol.136, pp.10, 2011, https://doi.org/10.1039/c0an00724b
  4. Ultrahigh-pressure dual online solid phase extraction/capillary reverse-phase liquid chromatography/tandem mass spectrometry (DO-SPE/cRPLC/MS/MS): A versatile separation platform for high-throughput and highly sensitive proteomic analyses vol.28, pp.6, 2007, https://doi.org/10.1002/elps.200600501
  5. A mitochondrial DNA variant at position 16189 is associated with type 2 diabetes mellitus in Asians vol.51, pp.4, 2008, https://doi.org/10.1007/s00125-008-0933-z
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  7. A Comprehensive Identification of Synaptic Vesicle Proteins in Rat Brains by cRPLC/MS-MS and 2DE/MALDI-TOF-MS vol.28, pp.9, 2004, https://doi.org/10.5012/bkcs.2007.28.9.1499