Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Determination of Glimepiride in Human Plasma by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

  • Kim, Ho-Hyun (Department of Pharmacokinetics, Seoul Medical Science Institute, Seoul Clinical Laboratories (SCL), Department of Drug Development Service, BioCore Co., Ltd) ;
  • Chang, Kyu-Young (Department of Drug Development Service, BioCore Co., Ltd) ;
  • Lee, Hee-Joo (Department of Pharmacokinetics, Seoul Medical Science Institute, Seoul Clinical Laboratories (SCL), Department of Drug Development Service, BioCore Co., Ltd) ;
  • Han, Sang-Beom (College of Pharmacy, ChungAng University)
  • Published : 2004.01.20
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Abstract

A sensitive method for quantitation of glimepiride in human plasma has been established using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Glipizide was used as an internal standard. Glimepiride and internal standard in plasma sample was extracted using diethyl etherethyl acetate (1 : 1). A centrifuged upper layer was then evaporated and reconstituted with the mobile phase of acetonitrile-5 mM ammonium acetate (60:40, pH 3.0). The reconstituted samples were injected into a $C_{18}$ reversed-phase column. Using MS/MS in the multiple reaction monitoring (MRM) mode, glimepiride and glipizide were detected without severe interference from human plasma matrix. Glimepiride produced a protonated precursor ion ([M+H]$^+$) at m/z 491 and a corresponding product ion at m/z 352. And the internal standard produced a protonated precursor ion ([M+H]]$^+$) at m/z 446 and a corresponding product ion at m/z 321. Detection of glimepiride in human plasma by the LC-ESI/MS/MS method was accurate and precise with a quantitation limit of 0.1 ng/mL. The validation, reproducibility, stability, and recovery of the method were evaluated. The method has been successfully applied to pharmacokinetic studies of glimepiride in human plasma.

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