Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Solution State Structure of pB1, the Mimotopic Peptide of Apolipoprotein B-100, by NMR

  • Lee, Sung-Ran (Department of Applied Chemistry, Hanyang University) ;
  • Kim, Dae-Sung (Department of Applied Chemistry, Hanyang University) ;
  • Kim, Hyo-Joon (Department of Biochemistry and Molecular Biology, Hanyang University) ;
  • Lee, Yong-Woo (R&D Center, Samsung Engineering Co. Ltd.) ;
  • Won, Ho-Shik (Department of Applied Chemistry, Hanyang University)
  • Published : 2004.12.20
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Abstract

Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1). From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.6 ${\AA}^2$ and the total RMSD was 0.5-1.5 ${\AA}. Moreover, pB1 structure included a weak $3_{10}$-helix from $Ile^7$,/TEX> to $Trp^{13}$.

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