The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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The Immunosuppressive Effect of Younggaechulgam-tang through Inhibition of Nuclear Translocation of Transcription Factor

전사인자의 핵 전이 억제를 통한 영계출감탕의 면역 억제 효과

  • 홍철희 (원광대학교 안이비인후피부과학교실) ;
  • 김남권 (원광대학교 안이비인후피부과학교실) ;
  • 이수형 (원광대학교 안이비인후피부과학교실) ;
  • 두인선 (원광대학교 안이비인후피부과학교실) ;
  • 황충연 (원광대학교 안이비인후피부과학교실)
  • Published : 20031100
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Abstract

Younggaechulgam-tang has been used for treating skin diseases. In this study, I investigated the immunosuppressive effect of Younggaechul-tang in the human T cell line MOLT-4 cells. MOLT-4 cells were stimulated with the phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) + A23187. The secretion appeared to be greater when cells were stimulated with PHA than with PMA + A23187. Younggaechulgam-tang had no affect proliferation stimulated by PHA. I showed that IL-2 secretion and expression by PHA stimulated MOLT-4 cells were inhibited by Younggaechugam-tang treatment. Maximal inhibition rate of IL-2, TNF-${\alpha}$ secretion was 80$\%$ and 30$\%$, respectively. Younggaechulgam-tang also inhibited nuclear translocation of p65 subunit of nuclear factor-kB and nuclear factor of activated T cells (NFAT). In conclusion, these results suggest that Younggaechulgam-tang may contribute to the immunosuppressive oriental drug clinically.

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