Clinical and Experimental Pediatrics
- Volume 46 Issue 6
- /
- Pages.554-560
- /
- 2003
- /
- 2713-4148(eISSN)
Experimental Study for the Teratogenic Effect of Gamma-ray on the Heart of Chick Embryo
계태에서 감마선 조사에 의한 심장기형 발생에 관한 실험적 연구
- Jo, Yong Whan (Department of Pediatrics, College of Medicine, Hanyang University) ;
-
Kim, Nam Su
(Department of Pediatrics, College of Medicine, Hanyang University) ;
- Moon, Sung Yup (Department of Pediatrics, College of Medicine, Hanyang University) ;
- Yum, Myeng Gul (Department of Pediatrics, College of Medicine, Hanyang University) ;
-
Kim, Sung Hoon
(Department of Radiotheraphy, College of Medicine, Hanyang University) ;
- Chun, Ha Chung (Department of Radiotheraphy, College of Medicine, Hanyang University) ;
-
Kim, Yong Joo
(Department of Pediatrics, College of Medicine, Hanyang University) ;
- Lee, Hahng (Department of Pediatrics, College of Medicine, Hanyang University)
- 조용환 (한양대학교 의과대학 소아과학교실) ;
-
김남수
(한양대학교 의과대학 소아과학교실) ;
- 문성엽 (한양대학교 의과대학 소아과학교실) ;
- 염명걸 (한양대학교 의과대학 소아과학교실) ;
-
김성훈
(한양대학교 의과대학 치료방사선과학교실) ;
- 전하정 (한양대학교 의과대학 치료방사선과학교실) ;
-
김용주
(한양대학교 의과대학 소아과학교실) ;
- 이항 (한양대학교 의과대학 소아과학교실)
- Received : 2003.12.04
- Accepted : 2003.04.11
- Published : 2003.06.15
Abstract
Purpose : To investigate the teratogenic effect of gamma-ray on the heart of chick embryo. Methods : 50 rad, 100 rad, 150 rad, 200 rad, 250 rad, and 300 rad of gamma-ray were used to irradiate three days old chick embryos. The control group was not irradiated. After three weeks, the embryos were sacrificed and examined for cardiovascular malformation. Results : The survival rate of the gamma-ray irradiated group was significantly lower than that of the control group(33.3-63.3% vs 76.4%, P=0.001). The cardiac malformation rate of the experimental group was 11.0%. In the control group, no congenital cardiac malformations were observed. The experimental groups had a significantly higher malformation rate(P=0.001). The types of malformation were ventricular septal defect, tricuspid atresia, Ebstein anomaly and aortic arch anomaly. In the gamma-ray irradiated group, the cardiac malformations were : 14 small ventricular septal defects (VSDs), five large VSDs, two tricuspid atresias, and one Ebstein anomaly. The higher the dose of radiation applied, the higher the incidence of cardiac malformation was noted. Conclusion : Gamma-ray irradiation of 3 days old chick embryos increased the rate of death and the rate of cardiac malformation significantly.
목 적 : 계태의 발생 과정에서 감마선 조사에 의한 심장 기형유발 유무와 심장 기형의 종류를 알아보기 위하여 본 연구를 시행하였다. 방 법 : 토종 닭의 수정란을 사서 닦은 후 대조군에게는 감마선을 조사하지 않았다. 실험군으로 닭의 수정란을 인공 부화기에 넣고 3일 후(72시간 후)에 꺼내어 700 curie 1.25 Mev Cobalt 60에서 나오는 감마선을 50 rad, 100 rad, 150 rad, 200 rad, 250 rad, 300 rad로 각각 60개의 수정란에 조사 후 인공 부화기에 넣어 총 3주간 지속시켰다. 온도