Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

The regulatory mechanism of insulin like growth factor secretion by high glucose in mesangial cell: involvement of cAMP

Mesangial 세포에서 고포도당에 의한 insulin-like growth factor의 분비조절기전에 관한 연구: cAMP와의 관련성

  • Heo, Jung-sun (Bio-safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kang, Chang-won (Bio-safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Han, Ho-jae (College of Veterinary Medicine, Hormone Research Center, Chonnam National University) ;
  • Park, Soo-hyun (Bio-safety Research Institute, College of Veterinary Medicine, Chonbuk National University)
  • 허정선 (전북대학교 수의과대학 생리학교실, 생체안전성 연구소) ;
  • 강창원 (전북대학교 수의과대학 생리학교실, 생체안전성 연구소) ;
  • 한호재 (전남대학교 수의과대학 생리학교실) ;
  • 박수현 (전북대학교 수의과대학 생리학교실, 생체안전성 연구소)
  • Accepted : 2003.11.20
  • Published : 2003.12.25
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Abstract

Dysfunction of mesangial cells has been contributed to the onset of diabetic nephropathy. Insulin like growth factors (IGFs) are also implicated in the pathogenesis of diabetic nephropathy. However, it is not yet known about the effect of high glucose on IGF-I and IGF-II secretion in the mesangial cells. Furthermore, the relationship between cAMP and high glucose on the secretion of IGFs was not elucidated. Thus, we examined the mechanisms by which high glucose regulates secretion of IGFs in mesangial cells. Glucose increased IGF-I secretion in a time- (>8 hr) and dose- (>15 mM) dependent manner (p<0.05). Stimulatory effect of high glucose on IGF-I secretion is predominantly observed in 25 mM glucose (high glucose), while 25 mM glucose did not affect cell viability and lactate dehydrogenase release. High glucose also increased IGF-II secretion. The increase of IGF-I and IGF-II secretion is not mediated by osmotic effect, since mannitol and L-glucose did not affect IGF-I and IGF-II secretion. 8-Br-cAMP mimicked high glucose-induced secretion of IGF-I and IGF-II. High glucose-induced stimulation of IGF-I and IGF-II secretion was blocked not by pertussis toxin but by SQ 22536 (adenylate cyclase inhibitor). Rp-cAMP (cAMP antagonist), and myristoylated protein kinase A (PKA) inhibitor amide 14-22 (protein kinase A inhibitor). These results suggest that cAMP/PKA pathways independent of Gi protein may mediate high glucose-induced increase of IGF-I and IGF-II secretion in mesangial cells. Indeed, glucose (>15 mM glucose) increased cAMP formation. In conclusion, high glucose stimulates IGF-I and IGF-II secretion via cAMP/PKA pathway in mesangial cells.

Keywords

References

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