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The Korean Association of Immunobiologists (대한면역학회)
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Production, Characterization, and Variable Region Analysis of Monoclonal Antibodies Specific for Hepatitis B Virus S Antigen

Hepatitis B Virus의 S항원에 특이적인 단세포군 항체 생산, 특성 연구 및 가변지역유전자 분석

  • Song, Moo-Young (Biotech Laboratory, Yuhan Research Institute, Yuhan Corporation) ;
  • Kim, Chang-Seok (Biotech Laboratory, Yuhan Research Institute, Yuhan Corporation) ;
  • Park, Sang-Koo (Biotech Laboratory, Yuhan Research Institute, Yuhan Corporation) ;
  • Lee, Jae-Sun (Biotech Laboratory, Yuhan Research Institute, Yuhan Corporation) ;
  • Yoo, Tae-Hyoung (Biotech Laboratory, Yuhan Research Institute, Yuhan Corporation) ;
  • Ko, In-Young (Biotech Laboratory, Yuhan Research Institute, Yuhan Corporation)
  • 송무영 (유한양행 중앙연구소 바이오텍 연구실) ;
  • 김창석 (유한양행 중앙연구소 바이오텍 연구실) ;
  • 박상구 (유한양행 중앙연구소 바이오텍 연구실) ;
  • 이재선 (유한양행 중앙연구소 바이오텍 연구실) ;
  • 유태형 (유한양행 중앙연구소 바이오텍 연구실) ;
  • 고인영 (유한양행 중앙연구소 바이오텍 연구실)
  • Published : 2003.12.30
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Abstract

Background: Hepatitis B virus (HBV) infection is one of the worldwide public health problem affecting about 300 million people. The envelope protein of HBV consists of three components known as preS1, preS2, and S antigen. According to the recent study, anti-HBs Ab showed effective neutralization ability against HBV from chronic hepatitis B and liver transplant patients, suggesting the possible development of therapeutic antibody. Methods: Spleen cells immunized with S antigen of HBV were fused with myeloma cell line to obtain HBsAg specific monoclonal antibodies. High affinity antibodies against HBsAg (adr, ad and ay type) were selected by competitive ELISA method. Nucleotide sequence of the variable regions of monoclonal antibodies was analyzed by RT-PCR followed by conventional sequencing method. Results: We produced 14 murine monoclonal antibodies which recognize S antigen of HBV. Two of them, A9-11 and C6-9 showed the highest affinity. The sequence analysis of A9-11 revealed that variable regions of the heavy chain and light chains are members of mouse heavy chain I (B) and light chain lambda 1, respectively. Likewise, the sequence analysis of C6-9 revealed that variable regions of the heavy chain and light chains are members of mouse heavy chain II (B) and light chain kappa 1, respectively. Neutralization assay showed that A9-11 and C6-9 effectively neutralize the HBV infection. Conclusion: These results suggest that A9-11 and C6-9 mouse monoclonal antibodies can be used for the development of therapeutic antibody for HBV infection.

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