대한치과보철학회지 (The Journal of Korean Academy of Prosthodontics)

  • 제41권6호
  • /
  • Pages.699-713
  • /
  • 2003
  • /
  • 0301-2875(pISSN)
  • /
  • 2005-3789(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
권호 표지

RESPONSE OF OSTEOBLASI-LIKE CELLS ON TITANIUM SURFACE TREATMENT

  • Roh Hyun-Ki (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Heo Seong-Joo (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Chang Ik-Tae (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Koak Jai-Young (Department of Prosthodontics, Graduate School, Seoul National University) ;
  • Han Jong-Hyun (Yonsei University, Yongdong Sevrance Hospital) ;
  • Kim Yong-Sik (University of Ulsan, Asan Medical Center) ;
  • Yim Soon-Ho (Sungkyunkwan University, School of Medicine)
  • 발행 : 2003.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Statement of problem. Titanium is the most important material for biomedical and dental implants because of their high corrosion resistance and good biocompatibility. These beneficial properties are due to a protective passive oxide film that spontaneously forms on the surface. Purpose. The purpose of this study was to evaluate the responses of osteoblast-like cells on different surface treatments on Ti discs. Material and Methods. Group 1 represented the machined surface with no treatment. Group 2 surfaces were sandblasted with $50{\mu}m\;Al_2O_3$ under $5kgf/cm^2$ of pressure. Groups 3 and 4 were sandblasted under the same conditions. The samples were treated on a titanium oxide surface with reactive sputter depositioning and thermal oxidation at $600^{\circ}C$ (Group 3) and $800^{\circ}C$ (Group 4) for one hour in an oxygen environment. The chemical composition and microtopography were analyzed by XRD, XPS, SEM and optical interferometer. The stability of $TiO_2$ layer was studied by petentiodynamic curve. To evaluate cell response, osteoblast extracted from femoral bone marrow of young adult rat were cultured for cell attachment, proliferation and morphology on each titanium discs. Results and Conclusion. The results were as follows : 1. Surface roughness values were, from the lowest to the highest, machined group, $800^{\circ}C$ thermal oxidation group, $600^{\circ}C$ thermal oxidation group and blasted group. The Ra value of blasted group was significantly higher than that of $800^{\circ}C$ thermal oxidation group (P=0.003), which was not different from that of $600^{\circ}C$ thermal oxidation group (P<0.05). 2. The degree of cell attachment was highest in the $600^{\circ}C$ thermal oxidation group after four and eight hours (P<0.05), but after 24 hours, there was no difference among the groups (P>0.05). 3. The level of cell proliferation showed no difference among the groups after one day, three days, and seven days (P>0.05). 4. The morphology and arrangement of the cells varied with surface roughness of the discs.

키워드

참고문헌

  1. Rich A, Harris AK. Anomalous preferences of cultures macrophages for hydrophobic and roughened substrata. J Cell Sci 1981; 50: 1-7
  2. Thomas K, Cook SD. An evaluationof variables influencing implant fixation and direct bone apposition. J Biomed Mater Res 1985;19:875-901 https://doi.org/10.1002/jbm.820190802
  3. Schroeder A, Van der Zypen E, Stich H, Sutter F. The reactions of bone, connective and epithelium to endosteal implants with titanium sprayed surfaces. J Maxillofac Surg 1981;9:15-25 https://doi.org/10.1016/S0301-0503(81)80007-0
  4. Buser D, Schenk R, Steinemann S, Riorellini J, Fox C, Stich H. Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs. J Biomed Mater Res 1991;25:889-902 https://doi.org/10.1002/jbm.820250708
  5. Cochran DL, Simpson J, Weber H, Buser D. Attachment and growth of periodontal cells on smooth and rough titanium. Int J Oral Maxillofac Implants 1994;9:289-976
  6. Michaels CM, Keller JC, Stanford CM, Solursh M. In vitro cell attachment of osteoblast-like cells to titanium. J Dent Res 1989;68:276
  7. Bowers KT, Keller JC, Randolph BA, Wick DG, Michaels CM. Optimization of surface micromorphology for enhanced osteoblast responses in vitro. Int J Oral Maxillofac Implants 1992 Fall;7(3):302-10
  8. Martin JY, Schwartz Z. Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). J Biomed Mater Res 1995 Mar;29(3):389-401 https://doi.org/10.1002/jbm.820290314
  9. Martin JY, Schwartz Z. Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). J Biomed Mater Res 1995 Mar;29(3):389-401 https://doi.org/10.1002/jbm.820290314
  10. Cheroudi B, Gould TRL, Brunette DM. Titaniumcoated micro-machined grooves of different dimensions affect epithelial and connective tissue cells differently in vivo. J Biomed Mater Res 1990;24:1203-19 https://doi.org/10.1002/jbm.820240906
  11. Brunette DM. The effects of implant surface topography on the behavior of cells. Int J Oral Maxillofac Implants 1988;3:231-46
  12. Golijanin L, Bernard G. Biocompatibility of implant metals in bone tissue culture. J Dent Res 1988;67:367
  13. Nowlin P, Carnes D, Windeler A. Biocompatibility of dental implant materials sputtered onto cell culture dishes. J Dent Res 1989;68:275
  14. Groessner-Schreiber b, Tuan RS. Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro. J Cell Sci 1992;101:209-17
  15. Ong JL, Prince CW, Raikar GN, Lucas LC. Effect of surface topography of titanium on surface chemistry and cellular response. Implant Dent 1996 Summer;5(2):83-8 https://doi.org/10.1097/00008505-199600520-00002
  16. Braun G, Kohavi D, Amir D, Luna MH, Caloss R, Sela J, Dean DD, Boyan BD, Schwartx X. Markers of primary mineralization are correlated with bone-bonding ability of titanium or stainless steel in vivo. Clin Oral Implants Res 1995;6:1-13 https://doi.org/10.1034/j.1600-0501.1995.060101.x
  17. Cooper LF, Masuda T, Whitson SW, Yliheikkila P, Felton DA. Formation of mineralizing osteoblast cultures on machined, titanium oxide grit-blasted, and plasma-sprayed titanium surfaces. Int J Oral Maxillofac Implants 1999 Jan-Feb;14(1):37-47
  18. Webb K, Hlady V, Tresco PA. Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization. J Biomed Mater Res 1998 Sep 5;41(3):422-30 https://doi.org/10.1002/(SICI)1097-4636(19980905)41:3<422::AID-JBM12>3.0.CO;2-K
  19. Kieswetter K, Schwartz Z. Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells. J Biomed Mater Res 1996 Sep;32(1):55-63 https://doi.org/10.1002/(SICI)1097-4636(199609)32:1<55::AID-JBM7>3.0.CO;2-O
  20. Lyndon f. Cooper, Takayufi Masuda. Formation of Mineralizing osteoblast cultures on machined, Titanium oxide Grit- Blasted, and plasma-sprayed titanium surfaces. Int J Oral Maxillofac Implants 1999;14:37-47
  21. Martin JY, Schwartz Z. Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63). J Biomed Mater Res 1995 Mar;29(3):389-401 https://doi.org/10.1002/jbm.820290314
  22. J.Lincks. Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition. Biomaterials 1998;19:2219-2232 https://doi.org/10.1016/S0142-9612(98)00144-6
  23. Adriano Piattelli. Histologic and Histom-orpho-metric Analysis of the Bone response to machined and sandblasted titanium implants: An experimental study in rabbits. Int J Oral Maxillofac Implants 1998;13:805-810
  24. Degasne I, Basle MF. Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2)on titanium surfaces. Calcif Tissue Int 1999 Jun;64(6): 499-507 https://doi.org/10.1007/s002239900640
  25. Bowers KT, Keller JC, Randolph BA, Wick DG, Michaels CM. Optimization of surface micromorphology for enhanced osteoblast responses in vitro. Int J Oral Maxillofac Implants 1992 Fall;7(3):302-10
  26. J.L.Ong. Surface roughness of titanium on bone morphogenetic protein-2 treated osteoblast cells in vitro. Implant Dent 1997;6:19-24
  27. Qu J, Chehroudi B, Brunette DM. The use of micromachined surfaces to investigate the cell behavioral factors essential to osseointegration. Oral Dis 1996 Mar;2(1):102-15 https://doi.org/10.1111/j.1601-0825.1996.tb00210.x
  28. Squire MW, Ricci JL, Bizios R. Analysis of osteoblast mineral deposits on orthopaedic/ dental implant metals. Biomaterials 1996 Apr;17(7):725-33 https://doi.org/10.1016/0142-9612(96)86743-3
  29. Norde W. Behavior of proteins at the interfaces,with special attention to the role of the structure stability of the protein molecule. Clin Mater 1998;11:85-91 https://doi.org/10.1016/0267-6605(92)90032-O
  30. Thomas CH, McFarland CD. The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry. J Biomed Mater Res 1997 Oct;37(1):81-93 https://doi.org/10.1002/(SICI)1097-4636(199710)37:1<81::AID-JBM10>3.0.CO;2-T
  31. Kieswetter K, Schwartz Z, Dean DD, Boyan BD.The role of implant surface characteristics in the healing of bone. Crit Rev Oral Biol Med 1996;7(4):329-45 https://doi.org/10.1177/10454411960070040301