Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Characterization of Biosurfactant Produced by Pseudomonas fluorescens PD101

Pseudomonos fluorescens PD101이 생산하는 생물유화제 특성

  • YOON Hong Mook (Department of Microbiology Pukyong National University) ;
  • MOON Sung Hoon (Department of Chemistry Pukyong National University) ;
  • SONG Young Hwan (Department of Microbiology Pukyong National University)
  • 윤홍묵 (부경대학교 자연과학대학 미생물학과) ;
  • 문성훈 (부경대학교 자연과학대학 화학과) ;
  • 송영환 (부경대학교 자연과학대학 미생물학과)
  • Published : 2003.06.01
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Abstract

Biosurfactant-producing bacteria, showing strong crude oil degrading activity, were isolated from the caverns of National Oil Storage Basement. From the results of biochemical and molecular biological tests, the isolate was identified as Pseudomonas fluorescens PD101. It grows well on liquid media at temperature range from $20^{\circ}C\;to\;37^{\circ}C,$ but it does not produce biosurfactant when grown at $37^{\circ}C$ or at higher temperature. The biosurfactant was stable at broad pH range from 5 to 11 and under heat treatment condition of $100^{\circ}C$ for 30 min. The biosurfactant produced dark blue halo around the colony when grown on SW agar plates, which could confirm the biosurfactant as one of rhamnolipid group. The 700 bp of PCR product could be amplified from DNA of P. flurorescens PD101 by using PCR primers designed from rh1A gene of P. aeruginosa, and it showed $99\%$ of sequence homology with rh1A gene of P. aeruginosa encoding rhamnosyltransferase 1.

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References

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